Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli and in in the PEP carboxykinase-deficient Saccharomyces cerevisiae-strain PUK-3B | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
F216Y | Km: 0.075 (MnADP-), 0.323 (phosphoenolpyruvate), 17 (HCO3-), 0.063 (Mn2+). Mutation Phe216Tyr shows that the introduction of a hydroxyl group in the lateral chain of the residue produces a substantial loss in the enzyme affinity for Mn2+, suggesting an increase of the pKa of Lys213 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
Mn2+ | - |
Saccharomyces cerevisiae | |
0.034 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
0.063 | - |
Mn2+ | mutant F216Y | Saccharomyces cerevisiae | |
0.075 | - |
ADP | mutant F216Y | Saccharomyces cerevisiae | |
0.075 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
0.307 | - |
phosphoenolpyruvate | - |
Saccharomyces cerevisiae | |
0.323 | - |
phosphoenolpyruvate | mutant F216Y | Saccharomyces cerevisiae | |
17 | - |
CO2 | mutant F216Y | Saccharomyces cerevisiae | |
17 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
18 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60980 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate + CO2 | - |
Saccharomyces cerevisiae | ATP + oxaloacetate | - |
r | |
ATP + oxaloacetate | - |
Saccharomyces cerevisiae | ADP + phosphoenolpyruvate + CO2 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PEP carboxykinase | - |
Saccharomyces cerevisiae |
phosphoenolpyruvate carboxykinase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
assay at | Saccharomyces cerevisiae |