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Literature summary for 4.1.1.49 extracted from

  • Jabalquinto, A.M.; Gonzalez-Nilo, F.D.; Laivenieks, M.; Cabezas, M.; Zeikus, J.G.; Cardemil, E.
    Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase. Mutagenesis at metal site 1 (2004), Biochimie, 86, 47-51.
    View publication on PubMed
Search for more literature for 4.1.1.49

Protein Variants

Protein Variants Comment Organism
D263E 16800fold reduction in maximal velocity compared to wild-type enzyme, KM-value for ADP is 1.8fold lower than wild-type value, KM-value for phosphoenolpyruvate is 1.3fold higher than wild-type value, KM-value for HCO3- is 1.1fold lower than wild-type value Anaerobiospirillum succiniciproducens
H225E 600fold reduction in maximal velocity compared to wild-type enzyme, KM-value for ADP is 2.4fold lower than wild-type value, KM-value for phosphoenolpyruvate is 2.6fold lower than wild-type value, KM-value for HCO3- is 1.1fold higher than wild-type value Anaerobiospirillum succiniciproducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 CO2 pH 6.5, 30°C, mutant enzyme D263E Anaerobiospirillum succiniciproducens
0.03
-
 CO2 CO2 in form of HCO3- Anaerobiospirillum succiniciproducens
0.032
-
 ADP pH 6.5, 30°C, mutant enzyme H225E Anaerobiospirillum succiniciproducens
0.033
-
 CO2 pH 6.5, 30°C, wild-type enzyme Anaerobiospirillum succiniciproducens
0.033
-
 CO2 CO2 in form of HCO3- Anaerobiospirillum succiniciproducens
0.036
-
 CO2 pH 6.5, 30°C, mutant enzyme H225E Anaerobiospirillum succiniciproducens
0.036
-
 CO2 CO2 in form of HCO3- Anaerobiospirillum succiniciproducens
0.043
-
 ADP pH 6.5, 30°C, mutant enzyme D263E Anaerobiospirillum succiniciproducens
0.077
-
 ADP pH 6.5, 30°C, wild-type enzyme Anaerobiospirillum succiniciproducens
0.085
-
 phosphoenolpyruvate pH 6.5, 30°C, mutant enzyme H225E Anaerobiospirillum succiniciproducens
0.219
-
 phosphoenolpyruvate pH 6.5, 30°C, wild-type enzyme Anaerobiospirillum succiniciproducens
0.289
-
 phosphoenolpyruvate pH 6.5, 30°C, mutant enzyme D263E Anaerobiospirillum succiniciproducens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ KM: 0.048 mM for wild-type enzyme, 0.038 mM for mutant enzyme H225E, 0.16 mM for mutant enzyme D263E Anaerobiospirillum succiniciproducens

Organism

Organism UniProt Comment Textmining
Anaerobiospirillum succiniciproducens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate + CO2
-
 Anaerobiospirillum succiniciproducens ATP + oxaloacetate
-
 ?