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Literature summary for 4.1.1.49 extracted from

  • Cristina Ravanal, M.; Flores, M.; Perez, E.; Aroca, F.; Cardemil, E.
    Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase: relevance of arginine 70 for catalysis (2004), Biochimie, 86, 357-362.
    View publication on PubMed
Search for more literature for 4.1.1.49

Protein Variants

Protein Variants Comment Organism
R70K 1.7fold increase in KM-value for ADP, 1.3fold increase in KM-value for phosphoenolpyruvate, 1.4fold increase in KM-value for HCO3- Saccharomyces cerevisiae
R70M 2.5fold increase in KM-value for ADP, 2.4fold increase in KM-value for phosphoenolpyruvate, 1.8fold decrease in KM-value for HCO3- Saccharomyces cerevisiae
R70Q 1.9fold increase in KM-value for ADP, 2.3fold increase in KM-value for phosphoenolpyruvate, 1.1fold increase in KM-value for HCO3- Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 CO2 pH 6.6, 30°C, mutant enzyme R70M Saccharomyces cerevisiae
0.01
-
 CO2 CO2 in form of HCO3- Saccharomyces cerevisiae
0.018
-
 CO2 pH 6.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
0.018
-
 CO2 CO2 in form of HCO3- Saccharomyces cerevisiae
0.019
-
 CO2 pH 6.6, 30°C, mutant enzyme R70Q Saccharomyces cerevisiae
0.019
-
 CO2 CO2 in form of HCO3- Saccharomyces cerevisiae
0.025
-
 CO2 pH 6.6, 30°C, mutant enzyme R70K Saccharomyces cerevisiae
0.025
-
 CO2 CO2 in form of HCO3- Saccharomyces cerevisiae
0.034
-
 ADP pH 6.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
0.058
-
 ADP pH 6.6, 30°C, mutant enzyme R70K Saccharomyces cerevisiae
0.063
-
 ADP pH 6.6, 30°C, mutant enzyme R70Q Saccharomyces cerevisiae
0.086
-
 ADP pH 6.6, 30°C, mutant enzyme R70M Saccharomyces cerevisiae
0.307
-
 phosphoenolpyruvate pH 6.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
0.41
-
 phosphoenolpyruvate pH 6.6, 30°C, mutant enzyme R70K Saccharomyces cerevisiae
0.72
-
 phosphoenolpyruvate pH 6.6, 30°C, mutant enzyme R70Q Saccharomyces cerevisiae
0.73
-
 phosphoenolpyruvate pH 6.6, 30°C, mutant enzyme R70M Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate + CO2 Arg70 is important for catalytic efficiency of the enzyme and for binding of phosphoenolpyruvate Saccharomyces cerevisiae ATP + oxaloacetate
-
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