Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.49 extracted from

  • Cymeryng, C.; Cazzulo, J.J.; Cannata, J.J.B.
    Phosphoenolpyruvate carboxykinase from Trypanosoma cruzi. Purification and physicochemical and kinetic properties (1995), Mol. Biochem. Parasitol., 73, 91-101.
    View publication on PubMed
Search for more literature for 4.1.1.49

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Trypanosoma cruzi
Fluorescein mercuric acetate
-
 Trypanosoma cruzi
IDP inhibits exchange reaction with ATP or GTP Trypanosoma cruzi
o-Iodosobenzoate 0.5 mM, 50% inhibition Trypanosoma cruzi
p-chloromercuribenzenesulfonate
-
 Trypanosoma cruzi
p-chloromercuribenzoate
-
 Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 ADP ADP in form of MnADP- Trypanosoma cruzi
0.019
-
 ATP exchange reaction Trypanosoma cruzi
0.019
-
 ATP ATP in form of MnATP2- Trypanosoma cruzi
0.027
-
 ATP ATP in form of MnATP2- Trypanosoma cruzi
0.035
-
 phosphoenolpyruvate
-
 Trypanosoma cruzi
0.041
-
 oxaloacetate exchange reaction Trypanosoma cruzi
0.044
-
 oxaloacetate
-
 Trypanosoma cruzi

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ exchange reaction: Mn2+ is the most effective divalent cation activator followed by Cd2+ and Mg2+ Trypanosoma cruzi
Mg2+ exchange reaction: MnCl2 is the most effective divalent cation activator followed by Cd2+ and Mg2+ Trypanosoma cruzi
Mn2+ exchange reaction: Mn2+ is the most effective divalent cation activator followed by Cd2+ and Mg2+ Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
 Tul O strain
-
Trypanosoma cruzi Tul O
-
 Tul O strain
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate + CO2
-
 Trypanosoma cruzi ATP + oxaloacetate
-
 ?
ADP + phosphoenolpyruvate + CO2
-
 Trypanosoma cruzi Tul O ATP + oxaloacetate
-
 ?
ATP + oxaloacetate
-
 Trypanosoma cruzi ADP + phosphoenolpyruvate + CO2
-
 ?
ATP + oxaloacetate
-
 Trypanosoma cruzi Tul O ADP + phosphoenolpyruvate + CO2
-
 ?
CTP + oxaloacetate 15% of the activity with ATP Trypanosoma cruzi CDP + phosphoenolpyruvate + CO2
-
 ?
GTP + oxaloacetate 52% of the activity with ATP Trypanosoma cruzi GDP + phosphoenolpyruvate + CO2
-
 ?
GTP + oxaloacetate 52% of the activity with ATP Trypanosoma cruzi Tul O GDP + phosphoenolpyruvate + CO2
-
 ?
ITP + oxaloacetate 36% of the activity with ATP Trypanosoma cruzi IDP + phosphoenolpyruvate + CO2
-
 ?
ITP + oxaloacetate 36% of the activity with ATP Trypanosoma cruzi Tul O IDP + phosphoenolpyruvate + CO2
-
 ?
additional information CO2-exchange reaction with ATP, GTP, ITP, CTP, UTP and TTP Trypanosoma cruzi ?
-
 ?
additional information CO2-exchange reaction with ATP, GTP, ITP, CTP, UTP and TTP Trypanosoma cruzi Tul O ?
-
 ?
TTP + oxaloacetate 16% of the activity with ATP Trypanosoma cruzi TDP + phosphoenolpyruvate + CO2
-
 ?
UTP + oxaloacetate 20% of the activity with ATP Trypanosoma cruzi UDP + phosphoenolpyruvate + CO2
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
 o-Iodosobenzoate
-
 Trypanosoma cruzi