KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
oxaloacetate | - |
Thermus thermophilus | |
0.04 | - |
ADP | - |
Thermus thermophilus | |
0.04 | - |
phosphoenolpyruvate | - |
Thermus thermophilus | |
0.08 | - |
ATP | - |
Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + oxaloacetate | Thermus thermophilus | the enzyme is neither regulatory nor inducible, the main function is catalyzing the synthesis of phosphoenopyruvate as a part of the gluconeogenetic pathway | ? | - |
? | |
ATP + oxaloacetate | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the enzyme is neither regulatory nor inducible, the main function is catalyzing the synthesis of phosphoenopyruvate as a part of the gluconeogenetic pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate + CO2 | - |
Thermus thermophilus | ATP + oxaloacetate | - |
? | |
ADP + phosphoenolpyruvate + CO2 | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ATP + oxaloacetate | - |
? | |
ATP + oxaloacetate | - |
Thermus thermophilus | ADP + phosphoenolpyruvate + CO2 | - |
? | |
ATP + oxaloacetate | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + phosphoenolpyruvate + CO2 | - |
? | |
ATP + oxaloacetate | the enzyme is neither regulatory nor inducible, the main function is catalyzing the synthesis of phosphoenopyruvate as a part of the gluconeogenetic pathway | Thermus thermophilus | ? | - |
? | |
ATP + oxaloacetate | the enzyme is neither regulatory nor inducible, the main function is catalyzing the synthesis of phosphoenopyruvate as a part of the gluconeogenetic pathway | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
50 min, stable | Thermus thermophilus |
90 | - |
about 50% loss of activity after 20 min, about 90% loss of activity after 45 min | Thermus thermophilus |
97 | - |
complete inactivation after 5 min | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
phosphoenolpyruvate formation, imidazole-HCl buffer | Thermus thermophilus |
7.5 | - |
phosphoenolpyruvate formation, Tris-HCl buffer | Thermus thermophilus |