Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
2.0 A structure | Saccharolobus solfataricus |
vapour diffusion method in hanging drops, the 2.0 A crystal structure is determined and compared with the known 2.0 A structure of the IGPS domain of the bifunctional enzyme from Escherichia coli. Both enzymes have only 30% sequence identity, but share high structural similarity | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
the higher stability of the enzyme from Sulfolobus solfataricus compared with the enzyme from E. coli seems to be the result of several improved interactions. Including a large number of salt bridges, stabilization of alpha-helices and strengthening of both polypeptide chain termini and solvent-exposed loops | Escherichia coli |
the higher stability of the enzyme from Sulfolobus solfataricus compared with the enzyme from E. coli seems to be the result of several improved interactions. Including a large number of salt bridges, stabilization of alpha-helices and strengthening of both polypeptide chain termini and solvent-exposed loops | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C | Saccharolobus solfataricus | |
0.09 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28600 | - |
x * 28600, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | Q06121 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Escherichia coli | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Saccharolobus solfataricus | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Escherichia coli | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? | |
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Saccharolobus solfataricus | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28600, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
eIGPS | - |
Escherichia coli |
indole-3-glycerol phosphate synthase | - |
Escherichia coli |
indole-3-glycerol phosphate synthase | - |
Saccharolobus solfataricus |
sIGPS | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
25 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the higher stability of the indole-3-glycerol phosphate synthase from Sulfolobus solfataricus compared to indole-3-glycerol phosphate synthase from Escherichia coli seems to be the result of several improved interactions. These include a larger number of salt bridges, stabilization of alpha helices and strengthening of both polypeptide chain termini and solvent-exposed loops | Escherichia coli |
additional information | - |
the higher stability of the indole-3-glycerol phosphate synthase from Sulfolobus solfataricus compared to indole-3-glycerol phosphate synthase from Escherichia coli seems to be the result of several improved interactions. These include a larger number of salt bridges, stabilization of alpha helices and strengthening of both polypeptide chain termini and solvent-exposed loops | Saccharolobus solfataricus |
44 | - |
half-life: 1.8 min | Escherichia coli |
89 | - |
half-life: 4.4 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C | Saccharolobus solfataricus | |
1.2 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.36 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C | Saccharolobus solfataricus | |
13.3 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C | Escherichia coli |