Protein Variants | Comment | Organism |
---|---|---|
L55S | Lys55Ser with 60fold decrease in turnover-number and 450fold increase in Km-value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type enzyme | Escherichia coli | |
0.54 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | mutant Lys55Ser | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | - |
Escherichia coli | 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | mutant enzyme Lys55Ser | Escherichia coli | |
7.2 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | wild-type enzyme | Escherichia coli |