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Literature summary for 4.1.1.46 extracted from

  • Zhang, X.; Ren, J.; Yao, P.; Gong, R.; Wang, M.; Wu, Q.; Zhu, D.
    Biochemical characterization and substrate profiling of a reversible 2,3-dihydroxybenzoic acid decarboxylase for biocatalytic Kolbe-Schmitt reaction (2018), Enzyme Microb. Technol., 113, 37-43 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Fusarium odoratissimum NRRL 54006

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: EDTA Fusarium odoratissimum NRRL 54006

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.2
-
2,3-Dihydroxybenzoate pH 6.0, 40°C Fusarium odoratissimum NRRL 54006
24.9
-
catechol pH 6.0, 40°C Fusarium odoratissimum NRRL 54006

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
143600
-
gel filtration Fusarium odoratissimum NRRL 54006

Organism

Organism UniProt Comment Textmining
Fusarium odoratissimum NRRL 54006 X0JT48
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes the decarboxylation of various substituted salicylic acids with different groups at varied positions except 5-position and the carboxylation of phenol and the substituted phenols Fusarium odoratissimum NRRL 54006 ?
-
?
2,3-Dihydroxybenzoate
-
Fusarium odoratissimum NRRL 54006 Catechol + CO2
-
r
catechol + CO2
-
Fusarium odoratissimum NRRL 54006 2,3-dihydroxybenzoate
-
r
3-fluoro-2-hydroxybenzoate
-
Fusarium odoratissimum NRRL 54006 2-fluorophenol + CO2
-
r
3-methoxy-6-hydroxybenzoate
-
Fusarium odoratissimum NRRL 54006 3-methoxyphenol + CO2
-
r
3-methoxyphenol + CO2
-
Fusarium odoratissimum NRRL 54006 3-methoxy-6-hydroxybenzoate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE Fusarium odoratissimum NRRL 54006

Synonyms

Synonyms Comment Organism
FOIG_04675
-
Fusarium odoratissimum NRRL 54006

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Fusarium odoratissimum NRRL 54006

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
2 h, 45% residual decarboxylase activity, 40% residual carboxylase activity Fusarium odoratissimum NRRL 54006
40
-
stable up to Fusarium odoratissimum NRRL 54006

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.78
-
catechol pH 6.0, 40°C Fusarium odoratissimum NRRL 54006
27.17
-
2,3-Dihydroxybenzoate pH 6.0, 40°C Fusarium odoratissimum NRRL 54006

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Fusarium odoratissimum NRRL 54006

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.031
-
catechol pH 6.0, 40°C Fusarium odoratissimum NRRL 54006
3.38
-
2,3-Dihydroxybenzoate pH 6.0, 40°C Fusarium odoratissimum NRRL 54006