Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
EPR spectroscopic study on the Cu-substituted enzyme and crystal structure in the native catalytically active state at 1.99 A resolution, a substrate analogue-bound form at 2.50 A resolution, and a selected active site mutant form with one of the putative substrate binding residues altered at 2.32 A resolution. Each asymmetric unit contains three pairs of dimers. The substrate analogue does not directly coordinate to the metal ion but is bound to the active site by two arginine residues through noncovalent interactions | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA, 1,10-phenanthroline or 8-hydroxyquinoline-5-sulfonic acid | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
2-aminomuconate semialdehyde | Cu-substituted enzyme, pH 7.0, temperature not specified in the publication | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the addition of one to ten equivalents of Co2+, Cu2+, Fe2+, or Zn2+ to purified ACMSD does not increase enzyme activity | Homo sapiens | |
Zn2+ | the activity of ACMSD is proportional to the zinc content of the enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TDX5 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
recombinant enzyme, pH 7.0, temperature not specified in the publication | Homo sapiens |
3.54 | - |
recombinant enzyme, presence of Zn2+ in expression medium, pH 7.0, temperature not specified in the publication | Homo sapiens |
Storage Stability | Organism |
---|---|
-78°C, enzyme endures freeze/thaw cycles multiple times without a significant loss of activity | Homo sapiens |
4°C, stable for weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminomuconate semialdehyde + CO2 | - |
Homo sapiens | 2-amino-3-(3-oxoprop-1-en-1-yl)but-2-enedioate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACMSD | - |
Homo sapiens |
alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2-aminomuconate semialdehyde | Cu-substituted enzyme, pH 7.0, temperature not specified in the publication | Homo sapiens | |
4.8 | - |
2-aminomuconate semialdehyde | enzyme with 64.4% zinc ion occupancy, pH 7.0, temperature not specified in the publication | Homo sapiens |