Literature summary for 4.1.1.45 extracted from

Pucci, L.; Perozzi, S.; Cimadamore, F.; Orsomando, G.; Raffaelli, N.
Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism (2007), FEBS J., 274, 827-840.

Search for more literature for 4.1.1.45

Application

Application	Comment	Organism
medicine	the enzyme is an therapeutic target for treating disorders associated with increased levels of tryptophan metabolites	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris GS115 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
H6A	activity decreases by about 82%	Homo sapiens
H8A	activity decreases by about 50%	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	0.5 mM, reduces the enzymatic activity to 25%	Homo sapiens
Cr3+	0.5 mM, reduces the enzymatic activity to 35%	Homo sapiens
Fe3+	0.1 mM, reduces the enzymatic activity to 15%	Homo sapiens
Kynurenic acid	1 mM, 59% residual activity	Homo sapiens
Picolinic acid	1 mM, 47% residual activity	Homo sapiens
quinolinic acid	1 mM, 61% residual activity	Homo sapiens
Zn2+	0.1 mM, reduces the enzymatic activity to 6%	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0065	-	2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate	in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	1.0 mM, increases activity to 130%	Homo sapiens
Fe2+	0.1 mM, increases activity to 124%	Homo sapiens
additional information	not influenced by 1 mM Mg2+, Mn2+, Ni2+ or Ca2+	Homo sapiens
additional information	the pure enzyme is 100% active in the absence of any metal ion	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	SDS-PAGE	Homo sapiens
50000	-	gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	plays a key role in tryptophan catabolism, the enzyme regulates NAD biosynthesis from the amino acid, directly affecting quinolinate and picolinate formation	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
hydroxyapatite column chromatography, MonoQ column chromatography and Superose 12 gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-
kidney	-	Homo sapiens	-
liver	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.006	-	crude cell extract, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C	Homo sapiens
1.39	-	after 231fold purification, in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C	Homo sapiens

Storage Stability

Storage Stability	Organism
4°C, 10 mM Tris-HCl, pH 8.0, 0.13 m NaCl, several weeks, remains stable	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate	-	Homo sapiens	2-aminomuconate-6-semialdehyde + CO2	-	?
additional information	plays a key role in tryptophan catabolism, the enzyme regulates NAD biosynthesis from the amino acid, directly affecting quinolinate and picolinate formation	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
2-amino 3-carboxymuconate 6-semialdehyde decarboxylase	-	Homo sapiens
ACMS decarboxylase	-	Homo sapiens
ACMSD I	enzymatically active form	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
-20	-	the purified protein is sensitive to freezing at -20°C	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1	-	2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate	in 50 mM 4-morpholinepropanesulfonic acid, pH 6.0, at 25°C	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	8	-	Homo sapiens

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Release 2023.1 (January 2023)