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Literature summary for 4.1.1.45 extracted from

  • Martynowski, D.; Eyobo, Y.; Li, T.; Yang, K.; Liu, A.; Zhang, H.
    Crystal structure of alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase: insight into the active site and catalytic mechanism of a novel decarboxylation reaction (2006), Biochemistry, 45, 10412-10421.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas fluorescens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 0.1 M Tris (pH 8.75), 15% PEG 5000, and 0.2 M MgCl2, at 20°C Pseudomonas fluorescens

Engineering

Protein Variants Comment Organism
H177A similar properties like the wild type enzyme Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0232
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate in 25 mM HEPES buffer pH 7.0, the enzyme contains Co2+ Pseudomonas fluorescens
0.0096
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate in 25 mM HEPES buffer pH 7.0, the enzyme contains Zn2+ Pseudomonas fluorescens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Pseudomonas fluorescens
Zn2+ native active site metal Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose HP column chromatography, Phenyl Sepharose column chromatography Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate
-
Pseudomonas fluorescens 2-aminomuconate-6-semialdehyde + CO2
-
?

Subunits

Subunits Comment Organism
monomer or dimer the protein exists in solution as a mixture of both monomer and dimer forms Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
ACMSD member of the metal-dependent amidohydrolase superfamily of the (beta/alpha)8 TIM barrel fold Pseudomonas fluorescens
alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase
-
Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.3
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate in 25 mM HEPES buffer pH 7.0, the enzyme contains Co2+ Pseudomonas fluorescens
6.5
-
2-amino-3-(3-oxoprop-2-enyl)-but-2-enedioate in 25 mM HEPES buffer pH 7.0, the enzyme contains Zn2+ Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Pseudomonas fluorescens