Application | Comment | Organism |
---|---|---|
analysis | the enzyme is a valuable tool for clinical analysis of ketone bodies in human plasma | Paenibacillus polymyxa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetylacetone | - |
Paenibacillus polymyxa | |
ClO4- | - |
Paenibacillus polymyxa | |
HSO3- | - |
Paenibacillus polymyxa | |
KCN | - |
Paenibacillus polymyxa | |
SCN- | - |
Paenibacillus polymyxa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
acetoacetate | - |
Paenibacillus polymyxa | |
400 | - |
acetoacetate | polymyxin MI | Paenibacillus polymyxa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Paenibacillus polymyxa | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
about 10 * 27000, SDS-PAGE | Paenibacillus polymyxa |
280000 | - |
gel filtration | Paenibacillus polymyxa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus polymyxa | - |
- |
- |
Paenibacillus polymyxa | - |
in addition to the intracellular enzyme, the extracellular produced polymyxin MI catalyzes the reaction | - |
Paenibacillus polymyxa A-57 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paenibacillus polymyxa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Paenibacillus polymyxa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
296 | - |
- |
Paenibacillus polymyxa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Acetoacetate | - |
Paenibacillus polymyxa | Acetone + CO2 | - |
? | |
Acetoacetate | - |
Paenibacillus polymyxa A-57 | Acetone + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | about 10 * 27000, SDS-PAGE | Paenibacillus polymyxa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, stable up to | Paenibacillus polymyxa |
80 | - |
30 min, complete inactivation | Paenibacillus polymyxa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
- |
Paenibacillus polymyxa |
6 | - |
polymyxin MI | Paenibacillus polymyxa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | pH 3: about 50% of maximal activity, pH 8: about 40% of maximal activity | Paenibacillus polymyxa |
5 | 8 | pH 5: about 40% of maximal activity, pH 8: about 25% of maximal activity | Paenibacillus polymyxa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | 4°C, 23 h, stable | Paenibacillus polymyxa |