Cloned (Comment) | Organism |
---|---|
RbcS is expressed in Oryza sativa cultivar Nipponbare | Sorghum bicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0217 | - |
CO2 | recombinant enzyme expressed in Oryza sativa transgenic line SS10, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C | Sorghum bicolor | |
0.0224 | - |
CO2 | recombinant enzyme expressed in Oryza sativa transgenic line SS16, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C | Sorghum bicolor | |
0.024 | - |
CO2 | recombinant enzyme expressed in Oryza sativa transgenic line SS5, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C | Sorghum bicolor | |
0.0415 | - |
CO2 | wild type enzyme, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
variant Tentaka | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Sorghum bicolor | 3-phospho-D-glycerate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rbcL | Rubisco large subunit | Sorghum bicolor |
rbcS | Rubisco small subunit | Sorghum bicolor |
Rubisco | - |
Sorghum bicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.22 | - |
CO2 | recombinant enzyme expressed in Oryza sativa transgenic line SS16, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C | Sorghum bicolor | |
2.48 | - |
CO2 | recombinant enzyme expressed in Oryza sativa transgenic line SS5, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C | Sorghum bicolor | |
2.52 | - |
CO2 | recombinant enzyme expressed in Oryza sativa transgenic line SS10, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C | Sorghum bicolor | |
4.09 | - |
CO2 | wild type enzyme, in 100 mM Bicine-NaOH, 1 mM EDTA, 5 mM MgCl2, 2 mM NaH2PO4, 0.4% (w/v) bovine serum albumin, 5 mM dithiothreitol, 4 mM amino-n-caproic acid, and 0.8 mM benzamidine, pH 8.0, at 28°C | Sorghum bicolor |
Organism | Comment | Expression |
---|---|---|
Sorghum bicolor | the overexpression of sorghum RbcS does not down-regulate the expression of RbcS gene family and stimulates the expression of RbcL in rice. In particular, the Rubisco content in SS5, a high expressing line, is 24% higher than that in nontransgenic rice | up |