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Literature summary for 4.1.1.39 extracted from
- Simultaneous determination of Rubisco carboxylase and oxygenase kinetic parameters in Triticum aestivum and Zea mays using membrane inlet mass spectrometry (2010), Plant Cell Environ., 33, 444-452.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | a membrane inlet mass spectrometer method is developed that simultaneously determines the rate of Rubisco carboxylation (vc) and oxygenation (vo) and the CO2 and O2 concentrations | Triticum aestivum |
| molecular biology | a membrane inlet mass spectrometer method is developped that simultaneously determines the rate of Rubisco carboxylation (vc) and oxygenation (vo) and the CO2 and O2 concentrations | Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0097 | - |
CO2 | Km (CO2): 0.291 mbar, V (oxygenation) max/V (carboxylation) max: 0.22, pH 7.95, 25°C | Triticum aestivum |  |
| 0.0162 | - |
CO2 | Km (CO2): 0.485 mbar, V (oxygenation) max/V (carboxylation) max: 0.11, pH 7.95, 25°C | Zea mays | |
| 0.183 | - |
O2 | Km (CO2): 0.146 mbar, pH 7.95, 25°C | Zea mays | |
| 0.244 | - |
O2 | Km (CO2): 0.194 mbar, pH 7.95, 25°C | Triticum aestivum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Triticum aestivum | |
| Mg2+ | - |
Zea mays | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Econo-Pac 10DG desalting column and filtration | Triticum aestivum |
| using a Econo-Pac 10DG desalting column and filtration | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Triticum aestivum | - |
| leaf | - |
Zea mays | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ribulose 1,5-bisphosphate + CO2 | - |
Triticum aestivum | ? | - |
? | |
| D-Ribulose 1,5-bisphosphate + CO2 | - |
Zea mays | ? | - |
? | |
| D-ribulose 1,5-bisphosphate + O2 | - |
Triticum aestivum | ? | - |
? | |
| D-ribulose 1,5-bisphosphate + O2 | - |
Zea mays | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ribulose 1,5-bisphosphate carboxylase/oxygenase | - |
Triticum aestivum |
| Ribulose 1,5-bisphosphate carboxylase/oxygenase | - |
Zea mays |
| Rubisco | - |
Triticum aestivum |
| Rubisco | - |
Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Triticum aestivum |
| 25 | - |
assay at | Zea mays |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
O2 | - |
Zea mays | |
| 0.83 | - |
O2 | - |
Triticum aestivum | |
| 3.8 | - |
CO2 | - |
Triticum aestivum | |
| 4.7 | - |
CO2 | - |
Zea mays | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.95 | - |
assay at | Triticum aestivum |
| 7.95 | - |
assay at | Zea mays |
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