Literature summary for 4.1.1.37 extracted from

Warby, C.A.; Phillips, J.D.; Bergonia, H.A.; Whitby, F.G.; Hill, C.P.; Kushner, J.P.
Structural and kinetic characterization of mutant human uroporphyrinogen decarboxylases (2009), Cell. Mol. Biol. (Noisy-le-grand), 55, 40-45.

Search for more literature for 4.1.1.37

Cloned(Commentary)

Cloned (Comment)	Organism
uroporphyrinogen decarboxylase mutations G318R, D306Y and K297N	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
recombinant mutant proteins K297N and G318R	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D306Y	mutation results in an insoluble recombinant protein	Homo sapiens
G318R	little effect on the structure or activity of recombinant uroporphyrinogen decarboxylase, but the protein displays reduced stability in vitro	Homo sapiens
K297N	little effect on the structure or activity of recombinant uroporphyrinogen decarboxylase, but the protein displays reduced stability in vitro	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0108	-	uroporphyrinogen-III	recombinant wild-type	Homo sapiens
0.0144	-	uroporphyrinogen-III	recombinant mutant K297N	Homo sapiens
0.0148	-	uroporphyrinogen-III	recombinant mutant G318R	Homo sapiens
0.0277	-	uroporphyrinogen-I	recombinant mutant G318R	Homo sapiens
0.033	-	uroporphyrinogen-I	recombinant mutant K297N	Homo sapiens
0.0381	-	uroporphyrinogen-I	recombinant wild-type	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uroporphyrinogen-III	Homo sapiens	-	coproporphyrinogen-III + 4 CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P06132	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
hepatocyte	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	recombinat mutant G318R substrate uroporphyrinogen-I 60.9 percent of recombinant wild-type	Homo sapiens
additional information	-	recombinat mutant G318R substrate uroporphyrinogen-III 76.2 percent of recombinant wild-type	Homo sapiens
additional information	-	recombinat mutant K297N substrate uroporphyrinogen-I 63.1 percent of recombinant wild-type	Homo sapiens
additional information	-	recombinat mutant K297N substrate uroporphyrinogen-III 73.7 percent of recombinant wild-type	Homo sapiens
additional information	-	subnormal uroporphyrinogen decarboxylase activity results in accumulation and urinary excretion of uroporphyrin and heptacarboxyl porphyrin	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
uroporphyrinogen-I	-	Homo sapiens	coproporphyrinogen-I + 4 CO2	-	?
uroporphyrinogen-III	-	Homo sapiens	coproporphyrinogen-III + 4 CO2	-	?

Synonyms

Synonyms	Comment	Organism
UroD	-	Homo sapiens
uroporphyrinogen decarboxylase	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.102	-	uroporphyrinogen-I	recombinant mutant G318R	Homo sapiens
0.104	-	uroporphyrinogen-I	recombinant mutant K297N	Homo sapiens
0.124	-	uroporphyrinogen-I	recombinant wild-type	Homo sapiens
0.317	-	uroporphyrinogen-III	recombinant wild-type	Homo sapiens
0.361	-	uroporphyrinogen-III	recombinant mutant K297N	Homo sapiens
0.425	-	uroporphyrinogen-III	recombinant mutant G318R	Homo sapiens

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Release 2023.1 (January 2023)