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Literature summary for 4.1.1.33 extracted from
- Bacopa monniera recombinant mevalonate diphosphate decarboxylase Biochemical characterization (2015), Int. J. Biol. Macromol., 79, 661-668 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacopa monnieri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.144 | - |
(R)-5-diphosphomevalonate | pH 8.0, temperature not specified in the publication | Bacopa monnieri |  |
| 1.386 | - |
ATP | pH 8.0, temperature not specified in the publication | Bacopa monnieri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimum activity in presence of Mg2+ | Bacopa monnieri | |
| additional information | divalent cation required | Bacopa monnieri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
MALDI-TOF | Bacopa monnieri |
| 94000 | - |
MALDI-TOF | Bacopa monnieri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacopa monnieri | A0A0F6WBJ2 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 36.7 | - |
pH 8.0, temperature not specified in the publication | Bacopa monnieri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20°C, pH 7.0, half-life 68 h | Bacopa monnieri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-5-diphosphomevalonate | - |
Bacopa monnieri | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 47000, calculated from sequence and SDS-PAGE, and monomer | Bacopa monnieri |
| monomer | 1 * 47000, calculated from sequence and SDS-PAGE, and dimer | Bacopa monnieri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacopa monnieri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
ATP | pH 8.0, temperature not specified in the publication | Bacopa monnieri | |
| 40 | - |
(R)-5-diphosphomevalonate | pH 8.0, temperature not specified in the publication | Bacopa monnieri | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacopa monnieri |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacopa monnieri | calculated from sequence | - |
6.2 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22 | - |
ATP | pH 8.0, temperature not specified in the publication | Bacopa monnieri | |
| 277 | - |
(R)-5-diphosphomevalonate | pH 8.0, temperature not specified in the publication | Bacopa monnieri | |
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Release 2023.1 (January 2023)
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