Crystallization (Comment) | Organism |
---|---|
molecular docking of inhibitor eriochrome black A, binds to the active site | Staphylococcus epidermidis |
Protein Variants | Comment | Organism |
---|---|---|
R193T | modest effects on kinetics | Staphylococcus epidermidis |
W153F | modest effects on kinetics | Staphylococcus epidermidis |
W19F | 130fold increase in Km for (R)-5-diphosphomevalonate, 10fold increase in Km for ATP | Staphylococcus epidermidis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
eriochrome black A | competitive with regard to (R)-5-diphosphomevalonate, inhibits both enzymatic activity and bacterial growth | Staphylococcus epidermidis | |
Eriochrome Black T | competitive with regard to (R)-5-diphosphomevalonate, inhibits both enzymatic activity and bacterial growth | Staphylococcus epidermidis | |
Eriochrome blue black | competitive with regard to (R)-5-diphosphomevalonate, inhibits both enzymatic activity and bacterial growth | Staphylococcus epidermidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0093 | - |
(R)-5-diphosphomevalonate | wild-type, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.015 | - |
ATP | mutant R193T, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.0267 | - |
ATP | wild-type, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.027 | - |
(R)-5-diphosphomevalonate | mutant W153F, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.038 | - |
(R)-5-diphosphomevalonate | mutant R193T, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.044 | - |
ATP | mutant W153F, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.3 | - |
ATP | mutant W19F, pH 7.0, 30°C | Staphylococcus epidermidis | |
1.3 | - |
(R)-5-diphosphomevalonate | mutant W19F, pH 7.0, 30°C | Staphylococcus epidermidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus epidermidis | Q9FD73 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.85 | - |
mutant W153F, pH 7.0, 30°C | Staphylococcus epidermidis |
3 | - |
mutant W19F, pH 7.0, 30°C | Staphylococcus epidermidis |
8.6 | - |
wild-type, pH 7.0, 30°C | Staphylococcus epidermidis |
14.4 | - |
mutant R193T, pH 7.0, 30°C | Staphylococcus epidermidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-diphosphomevalonate | - |
Staphylococcus epidermidis | ADP + phosphate + isopentenyl diphosphate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MvaD | - |
Staphylococcus epidermidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.14 | - |
(R)-5-diphosphomevalonate | mutant W153F, pH 7.0, 30°C | Staphylococcus epidermidis | |
1.8 | - |
(R)-5-diphosphomevalonate | mutant W19F, pH 7.0, 30°C | Staphylococcus epidermidis | |
5.3 | - |
(R)-5-diphosphomevalonate | wild-type, pH 7.0, 30°C | Staphylococcus epidermidis | |
8.8 | - |
(R)-5-diphosphomevalonate | mutant R193T, pH 7.0, 30°C | Staphylococcus epidermidis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00058 | - |
eriochrome black A | wild-type, substrate (R)-5-diphosphomevalonate, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.00059 | - |
Eriochrome Black T | wild-type, substrate ATP, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.0012 | - |
Eriochrome Black T | wild-type, substrate (R)-5-diphosphomevalonate, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.0018 | - |
eriochrome black A | wild-type, substrate ATP, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.0027 | - |
Eriochrome blue black | wild-type, substrate (R)-5-diphosphomevalonate, pH 7.0, 30°C | Staphylococcus epidermidis | |
0.0068 | - |
Eriochrome blue black | wild-type, substrate ATP, pH 7.0, 30°C | Staphylococcus epidermidis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0035 | - |
wild-type, pH 7.0, 30°C | Staphylococcus epidermidis | Eriochrome Black T | |
0.0035 | - |
wild-type, pH 7.0, 30°C | Staphylococcus epidermidis | Eriochrome blue black | |
0.005 | - |
wild-type, pH 7.0, 30°C | Staphylococcus epidermidis | eriochrome black A |