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Literature summary for 4.1.1.33 extracted from

  • Barta, M.L.; Skaff, D.A.; McWhorter, W.J.; Herdendorf, T.J.; Miziorko, H.M.; Geisbrecht, B.V.
    Crystal structures of Staphylococcus epidermidis mevalonate diphosphate decarboxylase bound to inhibitory analogs reveal new insight into substrate binding and catalysis (2011), J. Biol. Chem., 286, 23900-23910.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus epidermidis

Crystallization (Commentary)

Crystallization (Comment) Organism
MDD bound to diphosphoglycolyl proline and 6-fluoromevalonate diphosphate, hanging drop vapor diffusion method, using 0.25 M sodium formate and 16% (w/v) PEG 3350, at 20°C Staphylococcus epidermidis

Protein Variants

Protein Variants Comment Organism
S192A the mutation decreases kcat by about 1000fold Staphylococcus epidermidis

Inhibitors

Inhibitors Comment Organism Structure
(R)-6-fluoromevalonate diphosphate
-
 Staphylococcus epidermidis
(R,S)-diphosphoglycolyl proline competitive inhibitor Staphylococcus epidermidis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 (R,S)-5-diphosphomevalonate mutant enzyme S192A, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
0.0091
-
 (R,S)-5-diphosphomevalonate wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
0.027
-
 ATP wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
0.07
-
 ATP mutant enzyme S192A, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Staphylococcus epidermidis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, gel filtration Staphylococcus epidermidis

Organism

Organism UniProt Comment Textmining
Staphylococcus epidermidis Q9FD73
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA-Sepharose column chromatography and Resource Q anion-exchange chromatography Staphylococcus epidermidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R,S)-5-diphosphomevalonate
-
 Staphylococcus epidermidis ADP + phosphate + isopentenyl diphosphate + CO2
-
 ir

Subunits

Subunits Comment Organism
homodimer 2 * 36000, gel filtration Staphylococcus epidermidis

Synonyms

Synonyms Comment Organism
MDD
-
 Staphylococcus epidermidis
mevalonate diphosphate decarboxylase
-
 Staphylococcus epidermidis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0058
-
 (R,S)-5-diphosphomevalonate mutant enzyme S192A, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
5.9
-
 (R,S)-5-diphosphomevalonate wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Staphylococcus epidermidis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000049
-
 (R)-6-fluoromevalonate diphosphate wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis
0.0043
-
 (R,S)-diphosphoglycolyl proline wild type enzyme, at 30°C in 100 mM HEPES-NaOH (pH 7.0) Staphylococcus epidermidis