Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
D75A | 75% reduced activity | Mycolicibacterium smegmatis |
D75N | reduced activity | Mycolicibacterium smegmatis |
D75Q | reduced activity | Mycolicibacterium smegmatis |
D75S | reduced activity | Mycolicibacterium smegmatis |
D78A | severely reduced activity | Mycolicibacterium smegmatis |
E83A | severely reduced activity | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
oxaloacetate | wild type enzyme | Mycolicibacterium smegmatis | |
0.0115 | - |
oxaloacetate | mutant enzyme E83A | Mycolicibacterium smegmatis | |
0.0138 | - |
oxaloacetate | mutant enzyme D75N | Mycolicibacterium smegmatis | |
0.018 | - |
oxaloacetate | mutant enzyme D75Q | Mycolicibacterium smegmatis | |
0.0183 | - |
oxaloacetate | mutant enzyme D75A | Mycolicibacterium smegmatis | |
0.0189 | - |
oxaloacetate | mutant enzyme D75S | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71200 | - |
calculated from the nucleic acid sequence-derived amino acid sequence | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | Q9AGJ6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
mutant enzyme D78A, phosphoenolpyruvate-forming activity in the presence of 0.2 mM Mn2+ | Mycolicibacterium smegmatis |
0.13 | - |
mutant enzyme D78A, oxaloacetate-forming activity in the presence of 0.2 mM Mn2+ | Mycolicibacterium smegmatis |
0.15 | - |
mutant enzyme D78A, oxaloacetate-forming activity in the presence of 2 mM Mn2+ | Mycolicibacterium smegmatis |
0.36 | - |
mutant enzyme E83A, phosphoenolpyruvate-forming activity in the presence of 0.2 mM Mn2+ | Mycolicibacterium smegmatis |
0.4 | - |
mutant enzyme E83A, oxaloacetate-forming activity in the presence of 0.2 mM Mn2+ | Mycolicibacterium smegmatis |
1 | - |
mutant enzyme E83A, oxaloacetate-forming activity in the presence of 2 mM Mn2+ | Mycolicibacterium smegmatis |
10.6 | - |
mutant enzyme D75A, oxaloacetate-forming activity in the presence of 0.2 mM Mn2+ | Mycolicibacterium smegmatis |
12.3 | - |
wild type enzyme, phosphoenolpyruvate-forming activity in the presence of 0.2 mM Mn2+ | Mycolicibacterium smegmatis |
15.5 | - |
mutant enzyme D75A, phosphoenolpyruvate-forming activity in the presence of 0.2 mM Mn2 | Mycolicibacterium smegmatis |
29.4 | - |
wild type enzyme, oxaloacetate-forming activity in the presence of 2 mM Mn2+ | Mycolicibacterium smegmatis |
43 | - |
wild type enzyme, oxaloacetate-forming activity in the presence of 0.2 mM Mn2+ | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + oxaloacetate | - |
Mycolicibacterium smegmatis | GDP + phosphoenolpyruvate + CO2 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GTP-PEPCK | - |
Mycolicibacterium smegmatis |
PEP carboxykinase | - |
Mycolicibacterium smegmatis |
phosphoenolpyruvate carboxykinase, GTP-dependent | - |
Mycolicibacterium smegmatis |