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Literature summary for 4.1.1.32 extracted from
- Purification and properties of mitochondrial phosphoenolpyruvate carboxykinase from liver of Squalus acanthias (1990), Arch. Biochem. Biophys., 280, 376-382.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
GDP | in presence of 0.0015 mM Mn2+ | Squalus acanthias |  |
| 0.034 | - |
GDP | in presence of 0.02 mM Mn2+ | Squalus acanthias | |
| 0.035 | - |
GDP | in presence of 0.0015 mM Mn2+ | Squalus acanthias | |
| 0.037 | - |
GDP | in presence of 0.002 mM Mn2+ | Squalus acanthias | |
| 0.91 | - |
phosphoenolpyruvate | in presence of 0.0035 mM Mn2+ | Squalus acanthias | |
| 0.92 | - |
phosphoenolpyruvate | in presence of 0.002 mM Mn2+ | Squalus acanthias | |
| 0.96 | - |
phosphoenolpyruvate | in presence of 0.02 mM Mn2+ | Squalus acanthias | |
| 1.33 | - |
phosphoenolpyruvate | in presence of 0.0013 mM Mn2+ | Squalus acanthias | |
| 15.7 | - |
CO2 | in presence of 0.002 mM Mn2+ | Squalus acanthias | |
| 15.7 | - |
CO2 | CO2 in form of HCO3- | Squalus acanthias | |
| 17 | - |
CO2 | in presence of 0.0035 mM Mn2+ | Squalus acanthias | |
| 17 | - |
CO2 | CO2 in form of HCO3- | Squalus acanthias | |
| 20.9 | - |
CO2 | in presence of 0.02 mM Mn2+ | Squalus acanthias | |
| 20.9 | - |
CO2 | CO2 in form of HCO3- | Squalus acanthias | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69500 | - |
1 * 69500, SDS-PAGE | Squalus acanthias |
| 71000 | - |
gel filtration | Squalus acanthias |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Squalus acanthias | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Squalus acanthias |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Squalus acanthias | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
- |
Squalus acanthias |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.2 M phosphate, DTT and glycerol, stable for 1 month | Squalus acanthias |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP + phosphoenolpyruvate + CO2 | - |
Squalus acanthias | GTP + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 69500, SDS-PAGE | Squalus acanthias |
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