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Literature summary for 4.1.1.32 extracted from

  • Pönsgen-Schmidt, E.; Schneider, T.; Hammer, U.; Betz, A.
    Comparison of phosphoenolpyruvate-carboxykinase from autotrophically and heterotrophically grown Euglena and its role during dark anaerobiosis (1988), Plant Physiol., 86, 457-462.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.32

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate competitive with respect to both oxaloacetate and phosphoenolpyruvate Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 oxaloacetate enzyme from autotrophically grown cells Euglena gracilis
0.022
-
 oxaloacetate enzyme from heterotrophically grown cells Euglena gracilis
0.056
-
 GDP enzyme from autotrophically grown cells Euglena gracilis
0.065
-
 GTP enzyme from autotrophically grown cells Euglena gracilis
0.12
-
 GDP enzyme from heterotrophically grown cells Euglena gracilis
0.141
-
 GTP enzyme from heterotrophically grown cells Euglena gracilis
0.8
-
 phosphoenolpyruvate enzyme from autotrophically grown cells Euglena gracilis
0.95
-
 phosphoenolpyruvate enzyme from heterotrophically grown cells Euglena gracilis
4.5
-
 CO2 enzyme from autotrophically grown cells Euglena gracilis
4.5
-
 CO2 CO2 in form of KHCO3 Euglena gracilis
7
-
 CO2 enzyme from heterotrophically grown cells Euglena gracilis
7
-
 CO2 CO2 in form of HCO3- Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000
-
 3 * 240000, enzyme from autotrophically grown cells, SDS-PAGE Euglena gracilis
247000
-
 2 * 247000, enzyme from heterotrophically grown cells, SDS-PAGE Euglena gracilis
550000
-
 enzyme from heterotrophically grown cells, gel filtration Euglena gracilis
761000
-
 enzyme from autotrophically grown cells, gel filtration Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-
Euglena gracilis 1224-5/9
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from autotrophically grown cells and heterotrophically grown cells Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture grown autotrophically with CO2 in the light. The yield is higher when an additional carbon source like glucose is added. Highest yields result if both glucose and CO2 are provided together in the dark. No activity in cells provided with CO2 alone and kept in the dark Euglena gracilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + phosphoenolpyruvate + CO2
-
 Euglena gracilis GTP + oxaloacetate
-
 r
GDP + phosphoenolpyruvate + CO2
-
 Euglena gracilis 1224-5/9 GTP + oxaloacetate
-
 r
GTP + oxaloacetate
-
 Euglena gracilis GDP + phosphoenolpyruvate + CO2
-
 ?
GTP + oxaloacetate
-
 Euglena gracilis 1224-5/9 GDP + phosphoenolpyruvate + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 247000, enzyme from heterotrophically grown cells, SDS-PAGE Euglena gracilis
trimer 3 * 240000, enzyme from autotrophically grown cells, SDS-PAGE Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 carboxylation, enzyme from autotrophically grown cells Euglena gracilis
7.6
-
 carboxylation, enzyme from heterotrophically grown cells Euglena gracilis
8.8
-
 decarboxylation, enzyme from autotrophically and heterotrophically grown cells Euglena gracilis