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Literature summary for 4.1.1.32 extracted from

  • Miyatake, K.; Ito, T.; Kitaoka, S.
    Subcellular location and some properties of phosphoenolpyruvate carboxykinase (PEPCK) in Euglena gracilis (1984), Agric. Biol. Chem., 48, 2139-2141.
No PubMed abstract available
Search for more literature for 4.1.1.32

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 oxaloacetate
-
 Euglena gracilis
0.05
-
 ITP
-
 Euglena gracilis
0.059
-
 GDP
-
 Euglena gracilis
0.065
-
 GTP
-
 Euglena gracilis
0.066
-
 CO2 CO2 in form of HCO3- Euglena gracilis
0.13
-
 phosphoenolpyruvate
-
 Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Euglena gracilis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Euglena gracilis
Co2+ Km: 0.055 mM Euglena gracilis
Mg2+ activates Euglena gracilis
Mg2+ maximal activity at 1 mM Euglena gracilis
Mg2+ Km: 0.043 mM Euglena gracilis
Mn2+ activates Euglena gracilis
Mn2+ maximal activity at 1 mM Euglena gracilis
Mn2+ Km: 0.045 mM Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + phosphoenolpyruvate + CO2
-
 Euglena gracilis GTP + oxaloacetate
-
 ?
GTP + oxaloacetate
-
 Euglena gracilis GDP + phosphoenolpyruvate + CO2
-
 ?
IDP + phosphoenolpyruvate + CO2
-
 Euglena gracilis ITP + oxaloacetate
-
 r
ITP + oxaloacetate
-
 Euglena gracilis IDP + phosphoenolpyruvate + CO2
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 carboxylation Euglena gracilis
8.5
-
 decarboxylation Euglena gracilis