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Literature summary for 4.1.1.32 extracted from
- The purification, characterization, and activation of phosphoenolpyruvate carboxykinase from chicken liver mitochondria (1982), J. Biol. Chem., 257, 5503-5514.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol facilitates loss of activity | Gallus gallus |
| dithiothreitol stabilizes | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (Z)-Phosphoenol-3-bromopyruvate | linear competitive | Gallus gallus |  |
| 2-oxoglutarate | above 5.0 mM, weak | Gallus gallus | |
| alpha-(Dihydroxyphosphinylmethyl) acrylic acid | weak linear competitive | Gallus gallus | |
| citrate | above 5.0 mM, weak | Gallus gallus | |
| DL-isocitrate | above 5.0 mM, weak | Gallus gallus | |
| malate | above 5.0 mM, weak | Gallus gallus | |
| oxalate | above 5.0 mM, weak | Gallus gallus | |
| succinate | above 5.0 mM, weak | Gallus gallus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
GDP | - |
Gallus gallus | |
| 0.013 | - |
IDP | - |
Gallus gallus | |
| 0.03 | - |
(Z)-3-fluorophosphoenolpyruvate | - |
Gallus gallus | |
| 0.042 | - |
GDP | GDP in form of MnGDP- | Gallus gallus | |
| 0.067 | - |
phosphoenolpyruvate | - |
Gallus gallus | |
| 0.13 | - |
ITP | - |
Gallus gallus | |
| 0.197 | - |
IDP | IDP in form of MnIDP- | Gallus gallus | |
| 19 | - |
CO2 | CO2 in form of HCO3- | Gallus gallus | |
| 40 | - |
ITP | ITP in form of MnITP2- | Gallus gallus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Gallus gallus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates | Gallus gallus | |
| additional information | no requirement for a monovalent cation | Gallus gallus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70500 | - |
1 * 70500, SDS-PAGE under reducing conditions | Gallus gallus |
| 75400 | - |
high speed equilibrium sedimentation | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP + (Z)-3-fluorophosphoenolpyruvate + CO2 | - |
Gallus gallus | ? | - |
? | |
| GDP + phosphoenolpyruvate + CO2 | - |
Gallus gallus | GTP + oxaloacetate | - |
r | |
| GTP + oxaloacetate | - |
Gallus gallus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
| IDP + phosphoenolpyruvate + CO2 | - |
Gallus gallus | ITP + oxaloacetate | - |
r | |
| ITP + oxaloacetate | - |
Gallus gallus | IDP + phosphoenolpyruvate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70500, SDS-PAGE under reducing conditions | Gallus gallus |
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Release 2023.1 (January 2023)
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