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Literature summary for 4.1.1.32 extracted from
- Purification and characterization of cytosol phosphoenolpyruvate carboxykinase from bullfrog (Rana catesbeiana) liver (1979), J. Biochem., 86, 71-78.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
oxaloacetate | pH 7.2 | Lithobates catesbeianus |  |
| 0.067 | - |
GTP | exchange reaction | Lithobates catesbeianus | |
| 0.2 | - |
ITP | exchange reaction | Lithobates catesbeianus | |
| 0.8 | - |
phosphoenolpyruvate | pH 7.2 | Lithobates catesbeianus | |
| 0.8 | - |
IDP | pH 6.3 | Lithobates catesbeianus | |
| 0.8 | - |
GDP | at pH 6.3 or at pH 7.2 | Lithobates catesbeianus | |
| 1 | - |
oxaloacetate | exchange reaction | Lithobates catesbeianus | |
| 1.6 | - |
GTP | pH 7.2 | Lithobates catesbeianus | |
| 2.5 | - |
IDP | pH 7.2 | Lithobates catesbeianus | |
| 4 | - |
ITP | pH 7.2 | Lithobates catesbeianus | |
| 8 | - |
CO2 | pH 6.3 | Lithobates catesbeianus | |
| 8 | - |
CO2 | CO2 in form of HCO3- | Lithobates catesbeianus | |
| 9.6 | - |
phosphoenolpyruvate | pH 6.3 | Lithobates catesbeianus | |
| 31 | - |
CO2 | exchange reaction | Lithobates catesbeianus | |
| 31 | - |
CO2 | CO2 in form of HCO3- | Lithobates catesbeianus | |
| 46 | - |
CO2 | pH 7.2 | Lithobates catesbeianus | |
| 46 | - |
CO2 | CO2 in form of HCO3- | Lithobates catesbeianus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Lithobates catesbeianus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | can replace Mn2+ in activation of oxaloacetate decarboxylation | Lithobates catesbeianus | |
| Mn2+ | absolutely required for phosphoenolpyruvate carboxylation | Lithobates catesbeianus | |
| Mn2+ | half-maximal activation: of phosphoenolpyruvate carboxylation at 2 mM, of oxaloacetate decarboxylation at 0.4 mM, of exchange reaction at 8 mM | Lithobates catesbeianus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
glycerol gradient centrifugation | Lithobates catesbeianus |
| 75000 | - |
1 * 75000, SDS-PAGE | Lithobates catesbeianus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lithobates catesbeianus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lithobates catesbeianus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Lithobates catesbeianus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP + phosphoenolpyruvate + CO2 | - |
Lithobates catesbeianus | GTP + oxaloacetate | - |
r | |
| GTP + oxaloacetate | - |
Lithobates catesbeianus | GDP + phosphoenolpyruvate + CO2 | - |
? | |
| IDP + phosphoenolpyruvate + CO2 | - |
Lithobates catesbeianus | ITP + oxaloacetate | - |
? | |
| ITP + oxaloacetate | - |
Lithobates catesbeianus | IDP + phosphoenolpyruvate + CO2 | - |
? | |
| additional information | catalyzes an exchange reaction between HCO3- and the beta-carboxyl group of oxaloacetate | Lithobates catesbeianus | ? | - |
? | |
| additional information | ADP is completely inactive in carboxylation of oxaloacetate | Lithobates catesbeianus | ? | - |
? | |
| UDP + phosphoenolpyruvate + CO2 | very low activity | Lithobates catesbeianus | UTP + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 75000, SDS-PAGE | Lithobates catesbeianus |
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