Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.31 extracted from

  • Del Prete, S.; De Luca, V.; Capasso, C.; Supuran, C.T.; Carginale, V.
    Recombinant thermoactive phosphoenolpyruvate carboxylase (PEPC) from Thermosynechococcus elongatus and its coupling with mesophilic/thermophilic bacterial carbonic anhydrases (CAs) for the conversion of CO2 to oxaloacetate (2016), Bioorg. Med. Chem., 24, 220-225 .
    View publication on PubMed
Search for more literature for 4.1.1.31

Application

Application Comment Organism
synthesis multienzyme system composed of recombinant phosphoenolpyruvate, in combination with mesophilic/thermophilic bacterial carbonic anhydrases, for converting CO2 into oxaloacetate. The catalytic procedure is in two steps: the conversion of CO2 into bicarbonate by carbonic anhydrase, followed by the carboxylation of phosphoenolpyruvate with bicarbonate, catalyzed by PEPC, with formation of oxaloacetate. When coupled with the extremely thermostable carbonic anhydrase from Sulphurhydrogenibium azorense, the production of oxaloacetate is achieved even at temperatures up to 60°C Thermosynechococcus vestitus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 phosphoenolpyruvate pH 8.3, 23°C Thermosynechococcus vestitus

Organism

Organism UniProt Comment Textmining
Thermosynechococcus vestitus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + HCO3-
-
 Thermosynechococcus vestitus phosphate + oxaloacetate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min, no loss of activity Thermosynechococcus vestitus
50
-
 15 min, 84% residual activity Thermosynechococcus vestitus
60
-
 15 min, 71% residual activity Thermosynechococcus vestitus