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Literature summary for 4.1.1.31 extracted from

  • Park, S.; Lee, W.; Kim, H.; Pack, S.P.; Lee, J.
    Characterization of phosphoenolpyruvate carboxylase from Oceanimonas smirnovii in Escherichia coli (2015), Appl. Biochem. Biotechnol., 177, 217-225 .
    View publication on PubMed
Search for more literature for 4.1.1.31

Cloned(Commentary)

Cloned (Comment) Organism
codon-optimized expression in Escherichia coli Oceanimonas smirnovii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.139
-
 HCO3- 23°C, pH not specified in the publication Oceanimonas smirnovii
1.22
-
 phosphoenolpyruvate 23°C, pH not specified in the publication Oceanimonas smirnovii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 32% of the activity with Mg2+ Oceanimonas smirnovii
Cu2+ 73% of the activity with Mg2+ Oceanimonas smirnovii
Mg2+ best activator Oceanimonas smirnovii
additional information 2.8% activity in absence of divalent cation Oceanimonas smirnovii

Organism

Organism UniProt Comment Textmining
Oceanimonas smirnovii WP_019935846
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.8
-
 23°C, pH not specified in the publication Oceanimonas smirnovii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + HCO3-
-
 Oceanimonas smirnovii phosphate + oxaloacetate
-
 ?

Subunits

Subunits Comment Organism
? x * 98000, SDS-PAGE Oceanimonas smirnovii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 2% residual activity Oceanimonas smirnovii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Oceanimonas smirnovii

pH Range

pH Minimum pH Maximum Comment Organism
5
-
 no residual activity Oceanimonas smirnovii