Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by D-glucose 6-phosphate | Clostridium perfringens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Clostridium perfringens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 1.25-1.5 M sodium malonate, pH 7.0, as precipitant | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Asp | L-Asp competitively inhibits the enzyme with respect to the substrate, Mg2+-phosphoenolpyruvate | Clostridium perfringens | |
malonate | weak inhibitor | Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
phosphoenolpyruvate | in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C | Clostridium perfringens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion | Clostridium perfringens | |
Mg2+ | required for activity, PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion | Clostridium perfringens | |
Mn2+ | PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | Q8XLE8 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography and gel filtration | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + HCO3- | - |
Clostridium perfringens | phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | X-ray crystallography, analytical ultracentrifugation, or sedimentation velocity analysis | Clostridium perfringens |
Synonyms | Comment | Organism |
---|---|---|
archaeal-type phosphoenolpyruvate carboxylase | - |
Clostridium perfringens |
PEPC | - |
Clostridium perfringens |
PepcA | - |
Clostridium perfringens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-Asp | in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C | Clostridium perfringens |