Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose 6-phosphate | - |
Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-aspartate | - |
Arabidopsis thaliana | |
L-malate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
phosphoenolpyruvate | isozyme PPC1, at pH 8.5 | Arabidopsis thaliana | |
0.18 | - |
phosphoenolpyruvate | phosphorylated isozyme PPC, at pH 7.3 | Arabidopsis thaliana | |
0.34 | - |
phosphoenolpyruvate | dephosphorylated isozyme PPC1, at pH 7.3 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute dependence | Arabidopsis thaliana | |
Mn2+ | absolute dependence | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
107000 | - |
4 * 107000, native enzyme, gel filtration | Arabidopsis thaliana |
430000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
cultivar Landsberg erecta | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | purified PPC1 is phosphorylated at Ser-11, in vivo phosphorylation of isoform PPC1 during phosphate stress also activates this enzyme at pH 7.3 by significantly lowering its Km(phosphoenolpyruvate) value and sensitivity to inhibition by L-malate and L-Asp, while increasing its activation by D-glucose-6-phosphate | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, butyl-Sepharose column chromatography, Fractogel EMD DEAE-650 colum chromatography, Mono Q column chromatography, and Superdex-200 gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
shoot | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.034 | - |
clarified extract, at pH 8.5 | Arabidopsis thaliana |
22.3 | - |
after 660fold purification, at pH 8.5 | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Arabidopsis thaliana | phosphate + oxaloacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 107000, native enzyme, gel filtration | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
PEP carboxylase | - |
Arabidopsis thaliana |
PEPC | - |
Arabidopsis thaliana |
PEPC1 | isozyme | Arabidopsis thaliana |
Phosphoenolpyruvate carboxylase | - |
Arabidopsis thaliana |
PPC1 | isozyme | Arabidopsis thaliana |
PPCK1 | isozyme | Arabidopsis thaliana |
PPCK2 | isozyme | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | PEPC activity at pH 7.0 is approximately 50% of that occurring at pH 8.5 | Arabidopsis thaliana |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.3 | - |
dephosphorylated isozyme PPC1 | Arabidopsis thaliana | L-malate | |
0.52 | - |
dephosphorylated isozyme PPC1 | Arabidopsis thaliana | L-aspartate | |
0.68 | - |
phosphorylated isozyme PPC1 | Arabidopsis thaliana | L-malate | |
1.14 | - |
phosphorylated isozyme PPC1 | Arabidopsis thaliana | L-aspartate |