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Literature summary for 4.1.1.31 extracted from

  • Dharmarajan, L.; Kraszewski, J.L.; Mukhopadhyay, B.; Dunten, P.W.
    Expression, purification and crystallization of an archaeal-type phosphoenolpyruvate carboxylase (2009), Acta Crystallogr. Sect. F, 65, 1193-1196.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
D-glucose 6-phosphate
-
Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
L-aspartate
-
Arabidopsis thaliana
L-malate
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
phosphoenolpyruvate isozyme PPC1, at pH 8.5 Arabidopsis thaliana
0.18
-
phosphoenolpyruvate phosphorylated isozyme PPC, at pH 7.3 Arabidopsis thaliana
0.34
-
phosphoenolpyruvate dephosphorylated isozyme PPC1, at pH 7.3 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Arabidopsis thaliana 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute dependence Arabidopsis thaliana
Mn2+ absolute dependence Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
107000
-
4 * 107000, native enzyme, gel filtration Arabidopsis thaliana
430000
-
gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
cultivar Landsberg erecta
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein purified PPC1 is phosphorylated at Ser-11, in vivo phosphorylation of isoform PPC1 during phosphate stress also activates this enzyme at pH 7.3 by significantly lowering its Km(phosphoenolpyruvate) value and sensitivity to inhibition by L-malate and L-Asp, while increasing its activation by D-glucose-6-phosphate Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, butyl-Sepharose column chromatography, Fractogel EMD DEAE-650 colum chromatography, Mono Q column chromatography, and Superdex-200 gel filtration Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
shoot
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.034
-
clarified extract, at pH 8.5 Arabidopsis thaliana
22.3
-
after 660fold purification, at pH 8.5 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Arabidopsis thaliana phosphate + oxaloacetate
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 107000, native enzyme, gel filtration Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
PEP carboxylase
-
Arabidopsis thaliana
PEPC
-
Arabidopsis thaliana
PEPC1 isozyme Arabidopsis thaliana
Phosphoenolpyruvate carboxylase
-
Arabidopsis thaliana
PPC1 isozyme Arabidopsis thaliana
PPCK1 isozyme Arabidopsis thaliana
PPCK2 isozyme Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8.5 PEPC activity at pH 7.0 is approximately 50% of that occurring at pH 8.5 Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.3
-
dephosphorylated isozyme PPC1 Arabidopsis thaliana L-malate
0.52
-
dephosphorylated isozyme PPC1 Arabidopsis thaliana L-aspartate
0.68
-
phosphorylated isozyme PPC1 Arabidopsis thaliana L-malate
1.14
-
phosphorylated isozyme PPC1 Arabidopsis thaliana L-aspartate