Literature summary for 4.1.1.31 extracted from

Moraes, T.F.; Plaxton, W.C.
Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (rapeseed) suspension cell cultures: implications for phosphoenolpyruvate carboxylase regulation during phosphate starvation, and the integration of glycolysis with nitrogen assimilation (2000), Eur. J. Biochem., 267, 4465-4476.

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Activating Compound

Activating Compound	Comment	Organism	Structure
glucose 6-phosphate	activates	Brassica napus

Inhibitors

Inhibitors	Comment	Organism	Structure
DL-isocitrate	-	Brassica napus
L-Asp	-	Brassica napus
L-Glu	-	Brassica napus
L-malate	-	Brassica napus
quercetin	-	Brassica napus
rutin	-	Brassica napus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	phosphoenolpyruvate	pH 8.4, 24°C, pH 7.3, 24°C, enzyme from cells grown in presence of phosphate or in absence of phosphate, assay in presence of glycerol	Brassica napus
0.15	-	phosphoenolpyruvate	pH 7.3, 24°C, pH 7.3, 24°C, enzyme from cells grown in absence of phosphate, assay in presence of glycerol	Brassica napus
0.15	-	phosphoenolpyruvate	pH 8.4, 24°C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol	Brassica napus
0.16	-	phosphoenolpyruvate	pH 8.4, 24°C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol	Brassica napus
0.17	-	phosphoenolpyruvate	pH 7.3, 24°C, pH 7.3, 24°C, enzyme from cells grown in presence of phosphate, assay in presence of glycerol	Brassica napus
0.41	-	phosphoenolpyruvate	pH 7.3, 24°C, enzyme from cells grown in presence of phosphate, assay in absence of glycerol	Brassica napus
0.42	-	phosphoenolpyruvate	pH 7.3, 24°C, enzyme from cells grown in absence of phosphate, assay in absence of glycerol	Brassica napus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	absolute dependence for a divalent cation, Km: 0.084 mM	Brassica napus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
104000	-	x * 104000, SDS-PAGE	Brassica napus
440000	-	gel filtration	Brassica napus

Organism

Organism	UniProt	Comment	Textmining
Brassica napus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	the enzyme may exist in a dephosphorylated form in cell grown in absence of phosphate and in cells grown in presence of phosphate	Brassica napus

Purification (Commentary)

Purification (Comment)	Organism
-	Brassica napus

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	-	Brassica napus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
37.2	-	-	Brassica napus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + CO2	-	Brassica napus	phosphate + oxaloacetate	-	?

Subunits

Subunits	Comment	Organism
?	x * 104000, SDS-PAGE	Brassica napus

Synonyms

Synonyms	Comment	Organism
PEPC	-	Brassica napus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	9.5	-	Brassica napus

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.3	8.4	activity at pH 7.3 is almost 80% of the activity at pH 8.4	Brassica napus

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Release 2023.1 (January 2023)