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Literature summary for 4.1.1.31 extracted from

  • Hoban, D.J.; Lyric, R.M.
    Phosphoenolpyruvate carboxylase of Thiobacillus thioparus. I. General properties (1975), Can. J. Biochem., 53, 875-880.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dioxane stimulates Thiobacillus thioparus

Inhibitors

Inhibitors Comment Organism Structure
Asp L-Asp, competitive Thiobacillus thioparus
malate competitive; L-malate Thiobacillus thioparus
phosphate orthophosphate is noncompetitive with phosphoenolpyruvate Thiobacillus thioparus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
phosphoenolpyruvate
-
Thiobacillus thioparus
0.89
-
HCO3-
-
Thiobacillus thioparus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km: 0.37 mM Thiobacillus thioparus

Organism

Organism UniProt Comment Textmining
Thiobacillus thioparus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thiobacillus thioparus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.32
-
-
Thiobacillus thioparus

Storage Stability

Storage Stability Organism
-18°C, 10 mM 2-mercaptoethanol, stable for several weeks Thiobacillus thioparus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + CO2
-
Thiobacillus thioparus phosphate + oxaloacetate
-
?
phosphoenolpyruvate + HCO3-
-
Thiobacillus thioparus phosphate + oxaloacetate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
tricine buffer Thiobacillus thioparus
7.4
-
Tris-HCl buffer Thiobacillus thioparus