Cloned (Comment) | Organism |
---|---|
gene undec1A, cloned from metagenome through sequence-based screening of uncultured soil microorganisms, DNA and amino acid sequence determination and analysis, recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS | unidentified prokaryotic organism |
Protein Variants | Comment | Organism |
---|---|---|
V81L/F240S/I250S/D266L | generation of mutant Undec1A-1180 by random mutagenxadesis through sequential error-prone PCR from a mutagenesis library. The mutant has 5.62fold highxader specific activity than wild-type Undec1A at 35°C and pH 7.0. The optimum pH of Undec1A-1180 does not change. Molecular docking results indicatxade that amino acid residues Ala235, Val237, Asp239, Ile267, Ala268, and Lys298 in mutant Unxaddec1A-1180 protein help recognize substrate molecules and catalyze the reaction with of L-cysteine sulfinic acid. Mutation efficiency analysis of different combinations of Mg2+ and Mn2+, overview | unidentified prokaryotic organism |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Lineweaver-Burk plots | unidentified prokaryotic organism | |
1.1 | - |
3-sulfino-L-alanine | pH 7.0, 35°C, recombinant mutant V81L/F240S/I250S/D266L enzyme | unidentified prokaryotic organism | |
1.56 | - |
3-sulfino-L-alanine | pH 7.0, 35°C, recombinant wild-type enzyme | unidentified prokaryotic organism |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-sulfino-L-alanine | unidentified prokaryotic organism | - |
hypotaurine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
unidentified prokaryotic organism | A0A2I9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography | unidentified prokaryotic organism |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-sulfino-L-alanine | - |
unidentified prokaryotic organism | hypotaurine + CO2 | - |
? | |
additional information | substrate specificity, overview. Substrate analogues L-cysteine, L-proline, L-alanine, L-glutamate, and L-asparagxadinate (at 5 mM) are also used as substrates by enzyme mutant Undec1A-1180, highest activity with L-cysteine, low activity with L-proline, no activity with L-alanine | unidentified prokaryotic organism | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CSD | - |
unidentified prokaryotic organism |
L-cysteine sulfinate decarboxylase | - |
unidentified prokaryotic organism |
undec1A | - |
unidentified prokaryotic organism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
recombinant His-tagged wild-type and mutant enzymes | unidentified prokaryotic organism |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.763 | - |
3-sulfino-L-alanine | pH 7.0, 35°C, recombinant wild-type enzyme | unidentified prokaryotic organism | |
1.476 | - |
3-sulfino-L-alanine | pH 7.0, 35°C, recombinant mutant V81L/F240S/I250S/D266L enzyme | unidentified prokaryotic organism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant His-tagged wild-type and mutant enzymes | unidentified prokaryotic organism |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
unidentified prokaryotic organism |
General Information | Comment | Organism |
---|---|---|
malfunction | the mutant Undec1A-1180 has 5.62fold highxader specific activity than wild-type Undec1A | unidentified prokaryotic organism |
additional information | structure homology modelling and molecular docking, overview | unidentified prokaryotic organism |
physiological function | CSD is the rate-limiting enzyme of taurine biosynthesis | unidentified prokaryotic organism |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
3-sulfino-L-alanine | pH 7.0, 35°C, recombinant wild-type enz.yme | unidentified prokaryotic organism | |
1.34 | - |
3-sulfino-L-alanine | pH 7.0, 35°C, recombinant mutant V81L/F240S/I250S/D266L enzyme | unidentified prokaryotic organism |