Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sulfhydryl reducing agents | required, e.g. dithiothreitol, 2-mercaptoethanol, reduced glutathione | Rattus norvegicus |
General Stability | Organism |
---|---|
pyridoxal 5'-phosphate protects against denaturation by heat or urea | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BH4- | - |
Rattus norvegicus | |
noradrenaline | reversed by addition of 0.1 mM pyridoxal 5'-phosphate | Rattus norvegicus | |
Penicillamine | reversed by addition of 0.1 mM pyridoxal 5'-phosphate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
cysteine sulfinate | - |
Rattus norvegicus | |
0.66 | - |
cysteic acid | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, SDS-PAGE | Rattus norvegicus |
100000 | - |
gel filtration, sucrose density gradient centrifugation, nondenaturing PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, with EDTA, pyridoxal 5'-phosphate and dithiothreitol, 3-5 mg of protein, 25-30% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cysteic acid | - |
Rattus norvegicus | 2-Aminoethane sulfonate + CO2 | - |
? | |
L-Cysteine sulfinate | - |
Rattus norvegicus | 2-Aminoethane sulfinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
thermal inactivation at | Rattus norvegicus |
59 | - |
thermal inactivation at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
L-cysteine sulfinic acid, cysteic acid | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | coenzyme | Rattus norvegicus |