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Literature summary for 4.1.1.25 extracted from

  • Nagy, L.; Hiripi, L.
    Role of tyrosine, DOPA and decarboxylase enzymes in the synthesis of monoamines in the brain of the locust (2002), Neurochem. Int., 41, 9-16.
    View publication on PubMed
Search for more literature for 4.1.1.25

Inhibitors

Inhibitors Comment Organism Structure
(2S)-2-amino-3-(3-hydroxyphenyl)-2-methylpropanoic acid IC50: 6.37 mM Locusta migratoria
(2S)-2-amino-3-(4-hydroxyphenyl)-2-methylpropanoic acid IC50: 9.4 mM Locusta migratoria
3-hydroxybenzyl-hydrazine potent inhibitor, IC50: 0.0004 mM Locusta migratoria
alpha-methyl-2,4-dihydroxyphenylalanine IC50: 1.8 mM Locusta migratoria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Locusta migratoria
0.007
-
 L-tyrosine pH 7, 32°C Locusta migratoria
0.117
-
 L-Dopa pH 7, 32°C Locusta migratoria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
370000
-
 gel filtration Locusta migratoria

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Tyrosine Locusta migratoria
-
 Tyramine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Locusta migratoria
-
 migratorioides R.F., locust
-

Purification (Commentary)

Purification (Comment) Organism
partial Locusta migratoria

Source Tissue

Source Tissue Comment Organism Textmining
brain cerebral ganglia Locusta migratoria
-
additional information enzyme activity decreases in the different ganglia in the following order: optic lobes, cerebral ganglia, thoracic ganglia, sub-oesophageal ganglion, abdominal ganglia Locusta migratoria
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Dopa 16times lower affinity for DOPA than for tyrosine, the decarboxylation of DOPA is inhibited in the presence of the cofactor pyridoxal 5’-phosphate by 50-60%, maximum activity towards DOPA in the absence of the cofactor Locusta migratoria dopamine + CO2
-
 ?
L-Tyrosine
-
 Locusta migratoria Tyramine + CO2
-
 ?
L-Tyrosine 16times higher affinity for tyrosine than for DOPA Locusta migratoria Tyramine + CO2
-
 ?
additional information not: 5-hydroxytryptophan Locusta migratoria ?
-
 ?

Synonyms

Synonyms Comment Organism
TYR decarboxylase
-
 Locusta migratoria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
 assay at Locusta migratoria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.2
-
 Locusta migratoria

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate cofactor, presence inhibits decarboxylation of DOPA by 50-60% Locusta migratoria

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0004
-
 potent inhibitor, IC50: 0.0004 mM Locusta migratoria 3-hydroxybenzyl-hydrazine
1.8
-
 IC50: 1.8 mM Locusta migratoria alpha-methyl-2,4-dihydroxyphenylalanine
6.37
-
 IC50: 6.37 mM Locusta migratoria (2S)-2-amino-3-(3-hydroxyphenyl)-2-methylpropanoic acid
9.4
-
 IC50: 9.4 mM Locusta migratoria (2S)-2-amino-3-(4-hydroxyphenyl)-2-methylpropanoic acid