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Literature summary for 4.1.1.25 extracted from

  • Moreno-Arribas, V.; Lonvaud-Funel, A.
    Tyrosine decarboxylase activity of Lactobacillus brevis IOEB 9809 isolated from wine and L. brevis ATCC 367 (1999), FEMS Microbiol. Lett., 180, 55-60.
    View publication on PubMed
Search for more literature for 4.1.1.25

General Stability

General Stability Organism
L-tyrosine and pyridoxal 5’-phosphate stabilize Levilactobacillus brevis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol strong inhibition Levilactobacillus brevis
Citric acid stronger inhibitory effect on strain ATCC 367 than on IOEB 9809 Levilactobacillus brevis
ethanol 12%, slight inhibition of activity in cell suspension, not: up to 10% Levilactobacillus brevis
glycerol strong inhibition Levilactobacillus brevis
Lactic acid stronger inhibitory effect on strain ATCC 367 than on IOEB 9809 Levilactobacillus brevis
tyramine competitive product inhibition, stronger effect on strain ATCC 367 than on IOEB 9809 Levilactobacillus brevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
 L-tyrosine pH 5, 25°C, ATCC 367, crude extract Levilactobacillus brevis
0.67
-
 L-tyrosine pH 5, 25°C, IOEB 9809, crude extract Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Tyrosine Levilactobacillus brevis
-
 Tyramine + CO2
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 12%, slight inhibition of activity in cell suspension, not: up to 10% Levilactobacillus brevis

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis
-
 strains IOEB 9809, isolated from wine, and ATCC 367
-

Storage Stability

Storage Stability Organism
-20°C, TDC from strain IOEB 9809, 0.2 M sodium acetate, pH 5, 0.2 mM pyridoxal 5’-phosphate, 3.6 mM L-tyrosine, at least 2 months, stable Levilactobacillus brevis
-20°C, TDC from strain IOEB 9809, 0.2 M sodium acetate, pH 5, 24 h, 60% loss of activity Levilactobacillus brevis
4°C, crude extract, progressive loss of activity Levilactobacillus brevis
4°C, TDC from strain IOEB 9809, 0.2 M sodium acetate, pH 5, 0.2 mM pyridoxal 5’-phosphate, 3.6 mM L-tyrosine, 15 days, 50% loss of activity Levilactobacillus brevis
4°C, TDC from strain IOEB 9809, 0.2 M sodium acetate, pH 5, 24 h, 85% loss of activity Levilactobacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Dopa preference of L-tyrosine over L-DOPA as substrate, strain IOEB 9809: 18% of activity of L-tyrosine decarboxylation, strain ATCC 367: 22% of activity of L-tyrosine decarboxylation Levilactobacillus brevis dopamine + CO2
-
 ?
L-Tyrosine
-
 Levilactobacillus brevis Tyramine + CO2
-
 ?
L-Tyrosine preference of L-tyrosine over L-DOPA as substrate Levilactobacillus brevis Tyramine + CO2
-
 ?
additional information not: histidine, lysine, phenylalanine, tryptophan, ornithine Levilactobacillus brevis ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Levilactobacillus brevis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 strain IOEB 9809, in presence of L-tyrosine and pyridoxal 5’-phosphate, half-life: 15 days Levilactobacillus brevis
25
-
 strain IOEB 9809, in presence of L-tyrosine and pyridoxal 5’-phosphate, half-life: 6 days Levilactobacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Levilactobacillus brevis

pH Range

pH Minimum pH Maximum Comment Organism
3 7 active in the pH-range Levilactobacillus brevis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate activates, activity is dependent on cofactor Levilactobacillus brevis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
8
-
 tyramine strain ATCC 367 Levilactobacillus brevis
13
-
 tyramine strain IOEB 9809 Levilactobacillus brevis