Protein Variants | Comment | Organism |
---|---|---|
D91A | inactive mutant | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-hydroxyUMP | a transition state analogue inhibitor, binding structure in complex with the enzyme, overview | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Saccharomyces cerevisiae | the enzyme is the most effective pure protein catalyst known in nature | UMP + CO2 | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
orotidine 5'-phosphate = UMP + CO2 | reaction mechanism involving the active site aspartate residue Asp91, electrostatic stress furnishes the majority of the catalytic driving force for OMP decarboxylation, ylide generation is not the rate-limiting step in the ODCase reaction | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the yeast enzyme does not exhibit a measurable affinity for a substrate analogue in which the labile carboxylate group is replaced by a cationic substituent, the apo and ligand-bound forms of the enzyme show distinct open and closed forms, the closed form is catalytically competent | Saccharomyces cerevisiae | ? | - |
? | |
Orotidine 5'-phosphate | the enzyme is the most effective pure protein catalyst known in nature | Saccharomyces cerevisiae | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | the decarboxylase shows an extremely fast rate acceleration, 1017-fold rate enhancement, ODCase associates weakly with the substrate in the ground state, but then tightens its grip as the altered substrate approaches the chemical transition state, yielding a complex with an estimated dissociation constant Ktx of over 10-24 M | Saccharomyces cerevisiae | UMP + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ODCase | - |
Saccharomyces cerevisiae |
OMP decarboxylase | - |
Saccharomyces cerevisiae |