Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes C155S and D96A/C155S in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C155S | more stable than wild-type enzyme | Saccharomyces cerevisiae |
D96A/C155S | inactive mutant protein | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-hydroxy UMP | inhibits activity with orotidine and orotidine 5'-phosphate, mutant enzyme C155S | Saccharomyces cerevisiae | |
Barbituric acid | mutant enzyme C155S | Saccharomyces cerevisiae | |
Orotidine | mutant enzyme C155S | Saccharomyces cerevisiae | |
phosphate | competitive, mutant enzyme C155S | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
Orotidine | pH 7.2, 23°C, mutant enzyme C155S | Saccharomyces cerevisiae | |
30 | - |
orotidine 5'-phosphate | value is higher than 30 mM, pH 7.2, 23°C, mutant enzyme C155S | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
orotidine | low decarboxylation activity | Saccharomyces cerevisiae | uridine + CO2 | - |
? | |
Orotidine 5'-phosphate | - |
Saccharomyces cerevisiae | UMP + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000007 | - |
orotidine 5'-phosphate | pH 7.2, 23°C, mutant enzyme C155S | Saccharomyces cerevisiae | |
22 | - |
Orotidine | pH 7.2, 23°C, mutant enzyme C155S | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
phosphate | mutant enzyme C155S | Saccharomyces cerevisiae |