Crystallization (Comment) | Organism |
---|---|
in complex with the inhibitor 6-azauridine 5-phosphate | Methanothermobacter thermautotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-azauridine 5'-phosphate | - |
Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, most proficient enzyme known | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metals are involved in catalysis | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | Methanothermobacter thermautotrophicus | catalyzes the final step in the de novo biosynthesis of UMP | UMP + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
orotidine 5'-phosphate = UMP + CO2 | mechanism, thermodynamic data | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Thioorotidine 5'-phosphate | 50% reduced kcat-value compared with orotidine 5'-phosphate as substrate | Methanothermobacter thermautotrophicus | 4-Thiouridine 5'-phosphate + CO2 | - |
? | |
additional information | not: 2-thioorotidine 5'-phosphate | Methanothermobacter thermautotrophicus | ? | - |
? | |
Orotidine 5'-phosphate | mechanism involving an equilibrium pre-protonation of orotidine 5'-phosphate C5 by the catalytic Lys-72 residue that greatly reduces the barrier to subsequent decarboxylation, Lys-72 is not critical for substrate binding | Methanothermobacter thermautotrophicus | UMP + CO2 | - |
? | |
Orotidine 5'-phosphate | catalyzes the final step in the de novo biosynthesis of UMP | Methanothermobacter thermautotrophicus | UMP + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 50% reduced kcat-value for 4-thioorotidine 5'-phosphate compared with orotidine 5'-phosphate as substrate | Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactors are involved in catalysis | Methanothermobacter thermautotrophicus |