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Literature summary for 4.1.1.23 extracted from

  • Lee, T.S.; Chong, L.T.; Chodera, J.D.; Kollman, P.A.
    An alternative explanation for the catalytic proficiency of orotidine 5'-phosphate decarboxylase (2001), J. Am. Chem. Soc., 123, 12837-12848.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with the inhibitor 6-azauridine 5’-phosphate Methanothermobacter thermautotrophicus

Inhibitors

Inhibitors Comment Organism Structure
6-azauridine 5'-phosphate
-
Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, most proficient enzyme known Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metals are involved in catalysis Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Orotidine 5'-phosphate Methanothermobacter thermautotrophicus catalyzes the final step in the de novo biosynthesis of UMP UMP + CO2
-
?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
orotidine 5'-phosphate = UMP + CO2 mechanism, thermodynamic data Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Thioorotidine 5'-phosphate 50% reduced kcat-value compared with orotidine 5'-phosphate as substrate Methanothermobacter thermautotrophicus 4-Thiouridine 5'-phosphate + CO2
-
?
additional information not: 2-thioorotidine 5'-phosphate Methanothermobacter thermautotrophicus ?
-
?
Orotidine 5'-phosphate mechanism involving an equilibrium pre-protonation of orotidine 5'-phosphate C5 by the catalytic Lys-72 residue that greatly reduces the barrier to subsequent decarboxylation, Lys-72 is not critical for substrate binding Methanothermobacter thermautotrophicus UMP + CO2
-
?
Orotidine 5'-phosphate catalyzes the final step in the de novo biosynthesis of UMP Methanothermobacter thermautotrophicus UMP + CO2
-
?

Subunits

Subunits Comment Organism
dimer
-
Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information 50% reduced kcat-value for 4-thioorotidine 5'-phosphate compared with orotidine 5'-phosphate as substrate Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
additional information no cofactors are involved in catalysis Methanothermobacter thermautotrophicus