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Literature summary for 4.1.1.23 extracted from

  • Smiley, J.A.; Saleh, L.
    Active site probes for yeast OMP decarboxylase: inhibition constants of UMP and thio-substituted UMP analogues and greatly reduced activity toward CMP-6-carboxylate (1999), Bioorg. Chem., 27, 297-306.
No PubMed abstract available
Search for more literature for 4.1.1.23

Protein Variants

Protein Variants Comment Organism
K93C absence of Zn2+ in mutant ODCase Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
2- thiouridine 5'-phosphate mixed inhibition Saccharomyces cerevisiae
4-thiouridine 5'-phosphate competitive inhibition, stronger inhibitor than UMP Saccharomyces cerevisiae
UMP competitive inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 orotidine 5'-phosphate pH 7, 25°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ mechanism may include participation of Zn2+, which interacts at O4 with the substrate Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Orotidine 5'-phosphate Saccharomyces cerevisiae
-
 UMP + CO2
-
 ?
Orotidine 5'-phosphate Saccharomyces cerevisiae BJ5424
-
 UMP + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae BJ5424
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
orotidine 5'-phosphate = UMP + CO2 catalytic mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Thioorotidine 5'-phosphate nearly equally effective as orotidine 5'-phosphate Saccharomyces cerevisiae 4-Thiouridine 5'-phosphate + CO2
-
 ?
4-Thioorotidine 5'-phosphate nearly equally effective as orotidine 5'-phosphate Saccharomyces cerevisiae BJ5424 4-Thiouridine 5'-phosphate + CO2
-
 ?
additional information not: 2-thioorotidine 5’-phosphate, undetectable activity with CMP-6-carboxylate and weak binding to ODCase Saccharomyces cerevisiae ?
-
 ?
additional information not: 2-thioorotidine 5’-phosphate, undetectable activity with CMP-6-carboxylate and weak binding to ODCase Saccharomyces cerevisiae BJ5424 ?
-
 ?
Orotidine 5'-phosphate
-
 Saccharomyces cerevisiae UMP + CO2
-
 ?
Orotidine 5'-phosphate catalytic mechanism involving protonation at O2 and a proposed Zn2+ interaction at O4, role of Lys-93 in catalysis Saccharomyces cerevisiae UMP + CO2
-
 ?
Orotidine 5'-phosphate
-
 Saccharomyces cerevisiae BJ5424 UMP + CO2
-
 ?
Orotidine 5'-phosphate catalytic mechanism involving protonation at O2 and a proposed Zn2+ interaction at O4, role of Lys-93 in catalysis Saccharomyces cerevisiae BJ5424 UMP + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information high catalytic proficiency Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information
-
 Saccharomyces cerevisiae
0.015
-
 4-thiouridine 5'-phosphate pH 7, 25°C Saccharomyces cerevisiae
0.043
-
 2-thiouridine 5'-phosphate pH 7, 25°C Saccharomyces cerevisiae
0.092
-
 UMP pH 7, 25°C Saccharomyces cerevisiae