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Literature summary for 4.1.1.23 extracted from

  • Miller, B.G.; Butterfoss, G.L.; Short, S.A.; Wolfenden, R.
    Role of enzyme-ribofuranosyl contacts in the ground state and transition state for orotidine 5'-phosphate decarboxylase: a role for substrate destabilization? (2001), Biochemistry, 40, 6227-6232.
    View publication on PubMed
Search for more literature for 4.1.1.23

Cloned(Commentary)

Cloned (Comment) Organism
ura3 gene, wild-type and mutant ODCase, expression in Escherichia coli SS6130 Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
complexed with the inhibitor 6-hydroxyuridine 5’-phosphate Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D37A 300fold reduced kcat/Km value Saccharomyces cerevisiae
T100A 60fold reduced kcat/Km value Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyuridine 5'-phosphate competitive inhibition, at higher concentrations Saccharomyces cerevisiae
6-azauridine 5'-phosphate competitive inhibition Saccharomyces cerevisiae
6-hydroxyuridine 5'-phosphate mode of binding Saccharomyces cerevisiae
D-ribose 5-phosphate
-
 Saccharomyces cerevisiae
poly(ADP-D-ribose)n-1
-
 Saccharomyces cerevisiae
ribose weak Saccharomyces cerevisiae
UMP competitive inhibition Saccharomyces cerevisiae
xanthosine 5'-phosphate competitive inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 orotidine 5'-phosphate pH 7.2, 25°C, wild-type enzyme Saccharomyces cerevisiae
0.0042
-
 orotidine 5'-phosphate pH 7.2, 25°C, D37A mutant Saccharomyces cerevisiae
0.0052
-
 2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, T100A mutant Saccharomyces cerevisiae
0.0054
-
 2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, wild-type enzyme Saccharomyces cerevisiae
0.0067
-
 orotidine 5'-phosphate pH 7.2, 25°C, T100A mutant Saccharomyces cerevisiae
0.01
-
 2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, D37A mutant Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble recombinant mutant ODCase Saccharomyces cerevisiae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information functions without metals Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Orotidine 5'-phosphate Saccharomyces cerevisiae catalyzes the final step of pyrimidine biosynthesis UMP + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P03962
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyorotidine 5'-phosphate less effective than orotidine 5’-phosphate Saccharomyces cerevisiae 2'-deoxyuridine 5'-phosphate + CO2
-
 ?
orotic acid extremely poor substrate Saccharomyces cerevisiae ?
-
 ?
Orotidine 5'-phosphate enzyme/active site structure, mode of substrate binding Saccharomyces cerevisiae UMP + CO2
-
 ?
Orotidine 5'-phosphate catalyzes the final step of pyrimidine biosynthesis Saccharomyces cerevisiae UMP + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0017
-
 2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, D37A mutant Saccharomyces cerevisiae
0.15
-
 2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, wild-type enzyme Saccharomyces cerevisiae
0.16
-
 2'-deoxyorotidine 5'-phosphate pH 7.2, 25°C, T100A mutant Saccharomyces cerevisiae
0.9
-
 orotidine 5'-phosphate pH 7.2, 25°C, D37A mutant Saccharomyces cerevisiae
7.2
-
 orotidine 5'-phosphate pH 7.2, 25°C, T100A mutant Saccharomyces cerevisiae
44
-
 orotidine 5'-phosphate pH 7.2, 25°C, wild-type enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information functions without cofactors Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000064
-
 6-azauridine 5'-phosphate pH 7.4, 25°C, wild-type enzyme Saccharomyces cerevisiae
0.00041
-
 xanthosine 5'-phosphate pH 7.4, 25°C, wild-type enzyme Saccharomyces cerevisiae
0.0077
-
 6-azauridine 5'-phosphate pH 7.4, 25°C, D37A mutant Saccharomyces cerevisiae
0.0094
-
 6-azauridine 5'-phosphate pH 7.4, 25°C, T100A mutant Saccharomyces cerevisiae
0.012
-
 xanthosine 5'-phosphate pH 7.4, 25°C, T100A mutant Saccharomyces cerevisiae
0.039
-
 xanthosine 5'-phosphate pH 7.4, 25°C, D37A mutant Saccharomyces cerevisiae
0.081
-
 D-ribose 5-phosphate pH 7.4, 25°C Saccharomyces cerevisiae
0.2
-
 UMP pH 7.4, 25°C, wild-type enzyme Saccharomyces cerevisiae
0.21
-
 UMP pH 7.4, 25°C, D37A mutant Saccharomyces cerevisiae
0.26
-
 2'-deoxyuridine 5'-phosphate pH 7.4, 25°C Saccharomyces cerevisiae
0.37
-
 UMP pH 7.4, 25°C, T100A mutant Saccharomyces cerevisiae
0.7
-
 poly(ADP-D-ribose)n-1 pH 7.4, 25°C Saccharomyces cerevisiae
37
-
 ribose pH 7.4, 25°C Saccharomyces cerevisiae