General Stability | Organism |
---|---|
glycerol, 50% v/v is an effective stabilizer of the orotidine-5'-phosphate decarboxylase domain at 4°C, -20°C or -70°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
bifunctional UMP synthase contains activities of EC 2.4.2.10 and EC 4.1.1.23 | - |
Purification (Comment) | Organism |
---|---|
preparation and isolation of the orotidine-5'-phosphate decarboxylase domain of UMP synthase | Mus musculus |
Storage Stability | Organism |
---|---|
-70°C, dilute buffer plus 2 mM dithiothreitol, purified UMP synthase activity retains orotidine-5'-phosphate decarboxylase activity for several months | Mus musculus |
0°C or -70°C, 5 mM potassium phosphate, pH 7.0, 2 mM dithiothreitol, rapid loss of orotidine-5'-phosphate decarboxylase activity of the orotidine-5'-phosphate decarboxylase domain of UMP synthase | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Orotidine 5'-phosphate | - |
Mus musculus | UMP + CO2 | - |
? |