Literature summary for 4.1.1.22 extracted from

Vaaler, G.L.; Snell, E.E.
Pyridoxal 5'-phosphate dependent histidine decarboxylase: overproduction, purification, biosynthesis of soluble site-directed mutant proteins, and replacement of conserved residues (1989), Biochemistry, 28, 7306-7313.

Search for more literature for 4.1.1.22

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Morganella morganii

Protein Variants

Protein Variants	Comment	Organism
H231F	mutant enzymes His231Phe and His231Arg are inactive	Morganella morganii
H231N	mutant enzyme His231Asn is 0.2% as active as the wild-type enzyme	Morganella morganii
H231Q	mutant His231Gln is 12% as active as the wild-type enzyme	Morganella morganii
H231R	mutant enzymes His231Phe and His231Arg are inactive	Morganella morganii
K232A	mutant enzyme Lys232Ala is inactive but retains ability to bind both pyridoxal 5'-phosphate and His efficiently	Morganella morganii
additional information	none of the four residues Met233, Cys230, Cys239 and Ser322 are essential for activity, altough all replacements reduce the activity of the enzyme significantly	Morganella morganii
S229A	mutant Ser229Ala or Ser229Cys are about 7% as active as the wild-type enzyme	Morganella morganii
S229C	mutant Ser229Ala or Ser229Cys are about 7% as active as the wild-type enzyme	Morganella morganii

Organism

Organism	UniProt	Comment	Textmining
Morganella morganii	-	-	-
Morganella morganii AM-15	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Morganella morganii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-His	-	Morganella morganii	Histamine + CO2	-	?
L-His	-	Morganella morganii AM-15	Histamine + CO2	-	?

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Release 2023.1 (January 2023)