Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Treponema denticola |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 14-16% (w/v) PEG 1000, 100 mM MgCl2, and 100 mM imidazole (pH 8.0) | Treponema denticola |
Protein Variants | Comment | Organism |
---|---|---|
T40N | the specific actzivity of the mutant is 20000fold lower than that of wild type enzyme | Treponema denticola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola | |
0.009 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 30°C | Treponema denticola | |
0.06 | - |
5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola | |
13 | - |
CO2 | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17189 | - |
8 * 17189, calculated from amino acid sequence | Treponema denticola |
17195 | - |
8 * 17195, ESI-MS | Treponema denticola |
184000 | - |
gel filtration | Treponema denticola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Treponema denticola | Q73PV9 | - |
- |
Purification (Comment) | Organism |
---|---|
streptomycin precipitation, ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration | Treponema denticola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxy-5-aminoimidazole ribonucleotide + H+ | - |
Treponema denticola | 5-aminoimidazole ribonucleotide + CO2 | - |
r | |
5-aminoimidazole ribonucleotide + CO2 | - |
Treponema denticola | 4-carboxy-5-aminoimidazole ribonucleotide + H+ | - |
r | |
additional information | the enzyme does not bind N5-carboxy-4-carboxy-5-aminoimidazole ribonucleotide | Treponema denticola | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 17189, calculated from amino acid sequence | Treponema denticola |
homooctamer | 8 * 17195, ESI-MS | Treponema denticola |
Synonyms | Comment | Organism |
---|---|---|
AIR carboxylase | - |
Treponema denticola |
class II PurE | - |
Treponema denticola |
PurE | - |
Treponema denticola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola | |
65 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 30°C | Treponema denticola | |
77 | - |
CO2 | 50 mM Tris-HCl (pH 8.0), at 30°C | Treponema denticola |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Treponema denticola | calculated from amino acid sequence | - |
7.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.4 | - |
5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 10°C | Treponema denticola | |
33 | - |
4-carboxy-5-aminoimidazole ribonucleotide | 50 mM Tris-HCl (pH 8.0), at 30°C | Treponema denticola |