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Literature summary for 4.1.1.2 extracted from

  • Lee, E.; Jeong, B.C.; Park, Y.H.; Kim, H.H.
    Expression of the gene encoding oxalate decarboxylase from Bacillus subtilis and characterization of the recombinant enzyme (2014), BMC Res. Notes, 7, 598 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TOP 10 cells Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxalate + H+ Bacillus subtilis
-
 formate + CO2
-
 ?
oxalate + H+ Bacillus subtilis 128
-
 formate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34714
-
-
Bacillus subtilis 128 O34714
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalate + H+
-
 Bacillus subtilis formate + CO2
-
 ?
oxalate + H+
-
 Bacillus subtilis 128 formate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
OXDC
-
 Bacillus subtilis
pBy recombinant gene name Bacillus subtilis
YvrK
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
O2 purified oxalate decarboxylase degrades more than 50% of oxalate when incubated with MnCl2 and sodium oxalate in atmospheric O2 Bacillus subtilis