Literature summary for 4.1.1.2 extracted from

Campomanes, P.; Kellett, W.F.; Easthon, L.M.; Ozarowski, A.; Allen, K.N.; Angerhofer, A.; Rothlisberger, U.; Richards, N.G.
Assigning the EPR fine structure parameters of the Mn(II) centers in Bacillus subtilis oxalate decarboxylase by site-directed mutagenesis and DFT/MM calculations (2014), J. Am. Chem. Soc., 136, 2313-2323.

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His6-tagged Mn(II)-substituted W132F enzyme mutant	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His6-tagged Mn(II)-substituted W132F enzyme mutant, hanging drop vapour diffusion method, mixing of 0.002 ml of 5 mg/ml protein in 100 mM Tris-HCl, pH 8.5, and 500 mM NaCl with.0.002 ml of well solution containing 100 mM Tris-HCl, pH 8.5, 2 M NaCl, and 10% PEG 6000, 17°C, 2 months, X-ray diffraction structure determination and analysis at 1.9-2.1 A resolution	Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
W132F	site-directed mutagenesis, the mutation causes structural changes in the N-terminal and C-terminal metal center, superimposing of the X-ray crystal structures of the Mn-containing wild-type enzyme, PDB ID 1UW8, and the Co-containing W132F enzyme mutant	Bacillus subtilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	dependent on, two Mn(II) centers located in the N- and C-terminal cupin domains of the enzyme, hydrogen bonding interaction between the side chains of a conserved Tryp132 and Glu101 coordinating Mn(II) in the N-terminal domain. Computational analysis of electronic properties of protein-bound Mn(II) ions using X-ray crystal protein structure with PDB ID 1UW8, quantum mechanical/molecular mechanical optimization and modeling, density functional theory and density functional theory/molecular mechanical calculations. Experimental and calculated fine structure parameters of wild-type and W132F mutant enzymes, overview	Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
oxalate + H+	Bacillus subtilis	-	formate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O34714	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged Mn(II)-substituted W132F enzyme mutant by nickel affinity chromatography, dialysis, and ultrafiltration	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
oxalate + H+	-	Bacillus subtilis	formate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
OXDC	-	Bacillus subtilis

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Release 2023.1 (January 2023)