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Literature summary for 4.1.1.2 extracted from

  • Tanner, A.; Bornemann, S.
    Bacillus subtilis YvrK is an acid-induced oxalate decarboxylase (2000), J. Bacteriol., 182, 5271-5273.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.2

Activating Compound

Activating Compound Comment Organism Structure
additional information pH-dependent induction in acidic growth media, particularly at pH 5, but not by oxalate Bacillus subtilis
O2 dioxygen-dependent reaction suggesting the role of a transition metal in catalysis Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bacillus subtilis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information presence of a metal ion, role of a transition metal in catalysis Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43407
-
 5 * 44000, SDS-PAGE, 5 * 43407, calculated from the amino acid sequence, pentamer, but it is possible, that YvrK is actually a hexamer Bacillus subtilis
44000
-
 5 * 44000, SDS-PAGE, 5 * 43407, calculated from the amino acid sequence, pentamer, but it is possible, that YvrK is actually a hexamer Bacillus subtilis
220000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxalate + H+ Bacillus subtilis oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation formate + CO2
-
 ?
oxalate + H+ Bacillus subtilis 168 oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation formate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis 168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1000fold Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus subtilis

Storage Stability

Storage Stability Organism
-20°C, frozen in liquid nitrogen, pH buffered at or near 7, stable Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalate + H+ acts exclusively on oxalate Bacillus subtilis formate + CO2
-
 ?
oxalate + H+ oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation Bacillus subtilis formate + CO2
-
 ?
oxalate + H+ acts exclusively on oxalate Bacillus subtilis 168 formate + CO2
-
 ?
oxalate + H+ oxalate-degrading enzyme, possibly involved in decarboxylative phosphorylation, YvrK could contribute to the raising of cytoplasmic pH when the organism encounters low values of pH in soil and rotting vegetation Bacillus subtilis 168 formate + CO2
-
 ?

Subunits

Subunits Comment Organism
pentamer 5 * 44000, SDS-PAGE, 5 * 43407, calculated from the amino acid sequence, pentamer, but it is possible, that YvrK is actually a hexamer Bacillus subtilis

Synonyms

Synonyms Comment Organism
More belongs to the cupin superfamily, bicupin Bacillus subtilis
YvrK
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
3 7.5 70% of maximum activity at pH 3, no activity at pH 7.5 Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis predicted value
-
 5.1
Bacillus subtilis determined using PhastSystem
-
 6.1