Any feedback?
Literature summary for 4.1.1.19 extracted from
- Biochemical and biophysical studies of Helicobacter pylori arginine decarboxylase, an enzyme important for acid adaptation in host (2018), IUBMB Life, 70, 658-669 .
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a target for drug design | Helicobacter pylori |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ADC, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Helicobacter pylori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C487A | site-directed mutagenesis, the mutant shows less than 10% of wild-type activity, but only slightly reduced temperature stability compared to wild-type | Helicobacter pylori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-difluoromethylarginine | DFMA, competitive inhibition | Helicobacter pylori |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics | Helicobacter pylori | |
| 3.4 | - |
L-arginine | pH 7.4, 37°C | Helicobacter pylori | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Helicobacter pylori | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for catalysis, divalent metal ions are essential for activity, the metal binding residues are conserved | Helicobacter pylori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 407000 | - |
purified recombinant His-tagged enzyme, gel filtration | Helicobacter pylori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine | Helicobacter pylori | - |
agmatine + CO2 | - |
? | |
| L-arginine | Helicobacter pylori ATCC 700392 / 26695 | - |
agmatine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | O25176 | i.e. Campylobacter pylori | - |
| Helicobacter pylori ATCC 700392 / 26695 | O25176 | i.e. Campylobacter pylori | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| difluoromethylornithine | over 90% activity compared to L-arginine | Helicobacter pylori | 1-(4-amino-5,5-difluoropentyl)guanidine + CO2 | - |
? | |
| difluoromethylornithine | over 90% activity compared to L-arginine | Helicobacter pylori ATCC 700392 / 26695 | 1-(4-amino-5,5-difluoropentyl)guanidine + CO2 | - |
? | |
| L-arginine | - |
Helicobacter pylori | agmatine + CO2 | - |
? | |
| L-arginine | preferred substrate | Helicobacter pylori | agmatine + CO2 | - |
? | |
| L-arginine | - |
Helicobacter pylori ATCC 700392 / 26695 | agmatine + CO2 | - |
? | |
| L-arginine | preferred substrate | Helicobacter pylori ATCC 700392 / 26695 | agmatine + CO2 | - |
? | |
| L-ornithine | about 90% activity compared to L-arginine | Helicobacter pylori | putrescine + CO2 | - |
? | |
| L-ornithine | about 90% activity compared to L-arginine | Helicobacter pylori ATCC 700392 / 26695 | putrescine + CO2 | - |
? | |
| N-omega-nitro-L-arginine methyl ester | over 80% activity compared to L-arginine | Helicobacter pylori | methyl formate + 1-(4-amino-5,5-difluoropentyl)guanidine + CO2 | - |
? | |
| N-omega-nitro-L-arginine methyl ester | over 80% activity compared to L-arginine | Helicobacter pylori ATCC 700392 / 26695 | methyl formate + 1-(4-amino-5,5-difluoropentyl)guanidine + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 68000, about, recombinant His-tagged enzyme, SDS-PAGE | Helicobacter pylori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADC | - |
Helicobacter pylori |
| HP0422 | - |
Helicobacter pylori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Helicobacter pylori |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | activity range, profile overview | Helicobacter pylori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 63.4 | - |
Tm of recombinant enzyme mutant C487A | Helicobacter pylori |
| 65.8 | - |
Tm of recombinant wild-type enzyme | Helicobacter pylori |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.92 | - |
L-arginine | pH 7.4, 37°C | Helicobacter pylori | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Helicobacter pylori |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 11 | activity range, profile overview | Helicobacter pylori |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | the cofactor binding residues are conserved | Helicobacter pylori | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.118 | - |
alpha-difluoromethylarginine | pH 7.4, 37°C | Helicobacter pylori | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | arginine decarboxylases belong to a family of fold III PLP (pyridoxal 5'-phosphate)-dependent decarboxylases | Helicobacter pylori |
| metabolism | L-arginine formed by ADC may be transported out from cytosol to extracellular milieu by a transporter protein, AdiC. The metabolism of L-arginine by ADC in Helicobacter pylori might be crucial for its survival in the acidic environment | Helicobacter pylori |
| additional information | Cys487, a conserved residue located at the active-site, is involved in the catalysis, with a pKa value of about 7.2. The homology model of the protein shows conserved alpha/beta TIM barrel and beta-sandwich domains, which are characteristic features of fold III decarboxylases. Cys487 has a marginal role in the stability. Helicobacter pylori ADC homology modeling and docking studies, overview | Helicobacter pylori |
| physiological function | the metabolism of L-arginine by ADC in Helicobacter pylori might be crucial for its survival in the acidic environment. To neutralize the pH of the stomach, it transports L-arginine (+1 charge) into the cell and agmatine (+2 charge) out of the cell, which results in the export of 1 proton during each turnover and thus the bacteria can survive in the acidic environment | Helicobacter pylori |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
