Cloned (Comment) | Organism |
---|---|
expressed in the T7 expression system as a cleavable poly-Histagged fusion construct in Escherichia coli strain Rosetta (DE3) pLysS | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 1 M lithium sulfate monohydrate, 0.1 M Na citrate tribasic dihydrate pH 5.6, and 0.5 M ammonium sulfate | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, HiTrap Q column chromatography, and DEAE Sepharose column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.151 | - |
crude extract, in 67 mM Tris-HCl (pH 7.5), 3.3 mM EDTA, 3.3 mM dithiothreitol, 0.04 mM pyridoxal 5'-phosphate | Escherichia coli |
2.8 | - |
purified enzyme, in 67 mM Tris-HCl (pH 7.5), 3.3 mM EDTA, 3.3 mM dithiothreitol, 0.04 mM pyridoxal 5'-phosphate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Escherichia coli | agmatine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Escherichia coli |
SpeA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
around 50°C | Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.4 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | calculated from amino acid sequence | - |
5.1 |