Application | Comment | Organism |
---|---|---|
drug development | the enzyme is an attractive target for drug design | Yersinia pestis |
Cloned (Comment) | Organism |
---|---|
gene speA, expression of His-tagged enzyme in Escherichia coli | Yersinia pestis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pre-steady-state kinetics of substrate, product, and inhibitor with ADC by single-wavelength stopped-flow spectroscopy, overview | Yersinia pestis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Yersinia pestis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | Yersinia pestis | ADC is the primary polyamine biosynthetic enzyme in the pathway | agmatine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pestis | Q8ZHG8 | gene speA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Yersinia pestis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-arginine = agmatine + CO2 | reaction mechanism via geminal diamine intermediate | Yersinia pestis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | ADC is the primary polyamine biosynthetic enzyme in the pathway | Yersinia pestis | agmatine + CO2 | - |
? | |
L-arginine | the enzyme exists in two different conformational states, one that binds ligand and one that does not | Yersinia pestis | agmatine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme exists in two different conformational states, one that binds ligand and one that does not, overview | Yersinia pestis |
Synonyms | Comment | Organism |
---|---|---|
ADC | - |
Yersinia pestis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Yersinia pestis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a fold III, pyridoxal 5'-phosphate-dependent enzyme | Yersinia pestis |