Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | included in enzyme assay | Paramecium bursaria Chlorella virus-1 |
Cloned (Comment) | Organism |
---|---|
expression as His-tag fusion protein in Escherichia coli | Paramecium bursaria Chlorella virus-1 |
Protein Variants | Comment | Organism |
---|---|---|
D296E | responsible for changes in substrate specificity | Paramecium bursaria Chlorella virus-1 |
E296D | increased Km and decreased turnover with L-Arg, minor effect on Km with L-ornithine, decreased turnover with L-ornithine | Paramecium bursaria Chlorella virus-1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-difluoromethylarginine | 1 mM causes almost complete inhibition after 30 min incubation, irreversible | Paramecium bursaria Chlorella virus-1 | |
alpha-difluoromethylornithine | 10 mM causes 74% inhibition after 30 min incubation, irreversible | Paramecium bursaria Chlorella virus-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
L-Arg | pH 8.2, 37°C | Paramecium bursaria Chlorella virus-1 | |
0.48 | - |
L-Arg | pH 9.0, 40°C | Paramecium bursaria Chlorella virus-1 | |
46 | - |
L-ornithine | pH 9.0, 40°C | Paramecium bursaria Chlorella virus-1 | |
180 | - |
L-ornithine | pH 8.2, 37°C | Paramecium bursaria Chlorella virus-1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | Paramecium bursaria Chlorella virus-1 | key enzyme in the biosynthesis of polyamines, key enzyme in the biosynthesis of putrescine, plays a role in stress response | agmatine + CO2 | - |
? | |
L-ornithine | Paramecium bursaria Chlorella virus-1 | - |
putrescine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paramecium bursaria Chlorella virus-1 | - |
enzyme displays ornithine decarboxylase as well as arginine decarboxylase activity | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Paramecium bursaria Chlorella virus-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Paramecium bursaria Chlorella virus-1 | agmatine + CO2 | - |
? | |
L-arginine | key enzyme in the biosynthesis of polyamines, key enzyme in the biosynthesis of putrescine, plays a role in stress response | Paramecium bursaria Chlorella virus-1 | agmatine + CO2 | - |
? | |
L-ornithine | - |
Paramecium bursaria Chlorella virus-1 | putrescine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PBCV-1 DC | - |
Paramecium bursaria Chlorella virus-1 |