Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysE | Yersinia pestis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, in the presence and absence of Mg2+ and pyridoxal 5-phosphate | Yersinia pestis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Yersinia pestis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
4 * 74000, crystal structure analysis | Yersinia pestis |
163100 | - |
apo-ADC, light-scattering analysis | Yersinia pestis |
178000 | - |
holo-ADC, light-scattering analysis | Yersinia pestis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | Yersinia pestis | key enzyme in the biosynthesis of polyamines, key enzyme in the biosynthesis of putrescine, plays a role in stress response | agmatine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pestis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Yersinia pestis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Yersinia pestis | agmatine + CO2 | - |
? | |
L-arginine | key enzyme in the biosynthesis of polyamines, key enzyme in the biosynthesis of putrescine, plays a role in stress response | Yersinia pestis | agmatine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | in solution at pH 8.0, light-scattering analysis | Yersinia pestis |
tetramer | 4 * 74000, crystal structure analysis | Yersinia pestis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Yersinia pestis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Yersinia pestis | - |
- |
4.9 |